...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnic and Histochemistry >Predicting small molecule fluorescent probe localization in living cells using QSAR modeling. 1. Overview and models for probes of structure, properties and function in single cells
【24h】

Predicting small molecule fluorescent probe localization in living cells using QSAR modeling. 1. Overview and models for probes of structure, properties and function in single cells

机译：使用QSAR建模预测活细胞中的小分子荧光探针定位。 1.单细胞结构，特性和功能探针的概述和模型

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Small molecule fluorochromes (synonyms: biosensors, chemosensors, fluorescent probes, vital stains) are widely used to investigate the structure, composition, physicochemical properties and biological functions of living cells, tissues and organisms. Selective entry and accumulation within particular cells and cellular structures are key processes for achieving these diverse objectives. Despite the complexities, probes routinely are applied using standard protocols, often without experimenter awareness of what factors that control accumulation and localization. The mechanisms of many such selective accumulations, however, now are known. Moreover, the influence of physicochemical properties of probes on their uptake and localization often can be defined numerically, hence predicted, using quantitative structure activity relations (QSAR) models with its required numerical structure parameters (or "descriptors"). The state of the art of this approach is described. Available QSAR models are summarized for uptake into cells and localization in the cytosol, endoplasmic reticulum, generic biomembranes, Golgi apparatus, lipid droplets, lysosomes/endosomes, mitochondria, eukaryotic nuclei (histones and DNA), plasma membrane, and ribosomal RNA (cytoplasmic and nucleolar). Integration of such core models to both aid understanding and troubleshooting of current fluorescent probes and to assist the design of novel probes is outlined and illustrated using case examples. Limitations and generic problems arising with this approach and comments on application of such approaches to xenobiotics other than probes, e.g., drugs and herbicides, together with a brief note about an alternative approach to prediction, are given.
机译：小分子荧光染料（同义词：生物传感器，化学传感器，荧光探针，活菌）被广泛用于研究活细胞，组织和生物体的结构，组成，理化特性和生物学功能。特定细胞和细胞结构内的选择性进入和积累是实现这些多样化目标的关键过程。尽管存在复杂性，但通常使用标准协议来应用探针，而实验人员通常在不了解控制积累和定位的因素的情况下。但是，现在已经知道许多这种选择性积累的机理。此外，通常可以使用定量结构活性关系（QSAR）模型及其所需的数值结构参数（或“描述符”），以数字方式定义探针的理化特性对其摄取和定位的影响，从而可以对其进行预测。描述了这种方法的现有技术。总结了可用的QSAR模型，这些模型可用于细胞吸收并定位于细胞质，内质网，通用生物膜，高尔基体，脂质滴，溶酶体/内体，线粒体，真核（核糖体和DNA），质膜和核糖体RNA（胞质和核糖体）。核仁）。使用案例举例概述和说明了此类核心模型的集成，以帮助理解和排除当前的荧光探针并帮助其设计新探针。给出了这种方法的局限性和一般性问题，以及对将这种方法应用于除探针（例如药物和除草剂）以外的异种生物的评论，并简要介绍了另一种预测方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号