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首页> 外文期刊>Clinical and experimental allergy : >Regulatory cells, cytokine pattern and clinical risk factors for asthma in infants and young children with recurrent wheeze.
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Regulatory cells, cytokine pattern and clinical risk factors for asthma in infants and young children with recurrent wheeze.

机译：婴儿和幼儿哮喘的调节细胞，细胞因子模式和临床风险因素进行复发喘息。

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BACKGROUND: Several risk factors for asthma have been identified in infants and young children with recurrent wheeze. However, published literature has reported contradictory findings regarding the underlying immunological mechanisms. OBJECTIVES: This study was designed to assess and compare the immunological status during the first 2 years in steroid-naive young children with >or= three episodes of physician-confirmed wheeze (n=50), with and without clinical risk factors for developing subsequent asthma (i.e. parental asthma or a personal history of eczema and/or two of the following: wheezing without colds, a personal history of allergic rhinitis and peripheral blood eosinophilia >4%), with age-matched healthy controls (n=30). METHODS: Peripheral blood CD4(+)CD25(+) and CD4(+)CD25(high) T cells and their cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4), GITR and Foxp3 expression were analysed by flow cytometry. Cytokine (IFN-gamma, TGF-beta and IL-10), CTLA-4 and Foxp3 mRNA expression were evaluated (real-time PCR) after peripheral blood mononuclear cell stimulation with phorbol 12-myristate 13-acetate (PMA) (24 h) and house dust mite (HDM) extracts (7th day). RESULTS: Flow cytometry results showed a significant reduction in the absolute number of CD4(+)CD25(high) and the absolute and percentage numbers of CD4(+)CD25(+)CTLA-4(+) in wheezy children compared with healthy controls. Wheezy children at a high risk of developing asthma had a significantly lower absolute number of CD4(+)CD25(+) (P=0.01) and CD4(+)CD25(high) (P=0.04), compared with those at a low risk. After PMA stimulation, CTLA-4 (P=0.03) and Foxp3 (P=0.02) expression was diminished in wheezy children compared with the healthy children. After HDM stimulation, CTLA-4 (P=0.03) and IFN-gamma (P=0.04) expression was diminished in wheezy children compared with healthy children. High-risk children had lower expression of IFN-gamma (P=0.03) compared with low-risk and healthy children and lower expression of CTLA-4 (P=0.01) compared with healthy children. CONCLUSIONS: Although our findings suggest that some immunological parameters are impaired in children with recurrent wheeze, particularly with a high risk for asthma, further studies are needed in order to assess their potential as surrogate predictor factors for asthma in early life.
机译：背景：婴儿和幼儿的哮喘有几种危险因素已经在经常性喘息。然而，公布的文献已经报道了关于潜在免疫机制的矛盾调查结果。目的：本研究旨在评估和比较患有>或=三次医生证实喘息（n = 50）的三次发作，且没有临床风险因素的临床风险因素的临床风险因素哮喘（即父母哮喘或湿疹的个人史和/或以下两项：没有感冒的喘息，过敏性鼻炎和外周血嗜酸性粒细胞的个人历史> 4％），具有年龄匹配的健康对照（n = 30）。方法：通过流式细胞仪分析外周血CD4（+）CD25（+）CD25（+）和CD4（+）CD25（高）T细胞及其细胞毒性T淋巴细胞相关抗原-4（CTLA-4），GITR和FOXP3表达。在用Phorbol 12-Myristerate 13-乙酸盐（PMA）后，评估细胞因子（IFN-Gamma，TGF-β和IL-10），CTLA-4和FOXP3 mRNA表达（实时PCR）在肺鼠12-醋酸盐（PMA）（24小时）和房屋粉尘（HDM）提取物（第7天）。结果：与健康对照相比，流式细胞术结果表明，与健康对照相比，在喘息儿童中的CD4（+）CD25（高）的绝对数量和CD4（+）CD25（+）CTLA-4（+）的绝对和百分比数。与低于低于那些相比，高哮喘发育哮喘的哮喘的喘息患者具有显着降低的CD4（+）CD25（+）（p = 0.01）和CD4（+）CD25（高）（P = 0.04）。风险。与健康儿童相比，PMA刺激后，CTLA-4（P = 0.03）和FoxP3（P = 0.02）表达减少。与健康儿童相比，HDM刺激后，CTLA-4（P = 0.03）和IFN-Gamma（P = 0.04）表达减少。与健康儿童相比，高危儿童的IFN-Gamma（p = 0.03）的表达较低，与低风险和健康的儿童和CTLA-4（P = 0.01）的表达相比。结论：虽然我们的研究结果表明，在经常性喘息的儿童中，一些免疫学参数患者，特别是哮喘风险高，需要进一步的研究，以便将其作为早期哮喘哮喘的替代预测因子的潜力进行进一步的研究。

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