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首页> 外文期刊>Comparative clinical pathology >In vitro and in vivo studies on the alpha-glucosidase inhibitory effects of the leaf extract of Carica papaya Linn
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In vitro and in vivo studies on the alpha-glucosidase inhibitory effects of the leaf extract of Carica papaya Linn

机译：体外和体内研究Carica Papaya Linn叶片提取物的α-葡萄糖苷酶抑制作用

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摘要

The search for novel alpha-glucosidase inhibitory agents is currently an important scientific endeavour to the biopharmaceutical companies due to their potentials to reduce postprandial hyperglycaemia in type 2 diabetic patients. The present research attempted to investigate the alpha-glucosidase inhibitory effects of the leaf extract of Carica papaya. Aqueous, methanol, ethyl acetate and n-hexane extracts of C. papaya leaf were subjected to in vitro alpha-glucosidase inhibitory studies, and methanol extract was the most potent (IC_50= 171.83 ±26.89 μg/mL). Consequently, the methanol extract was further fractionated by column chromatography which yielded six pooled fractions (A-F) and fraction A (FA) showed the lowest IC_50 (78.62 ± 25.23 μg/mL). Subsequently, the FA was subjected to in vivo oral maltose and sucrose tolerance tests which revealed that FA had potent inhibitory effects on maltose and sucrose tolerance in rats. The computed area under the curves (AUC_(0-120)) further indicates that FA (200 mg/kg bw) have better inhibitory activity on maltase than sucrase. The GC-MS analysis of FA indicates the presence of phenolics, various fatty acids and their derivatives. This research suggests that the leaf extract of C. papaya possesses alpha-glucosidase inhibitory effects and might be exploited in retarding postprandial hyperglycaemia.
机译：寻求新型α-葡萄糖苷酶抑制剂是目前对生物制药公司的重要科学努力，由于其潜在的2型糖尿病患者减少了2型糖尿病患者。目前研究试图探讨Carica番木瓜叶子提取物的α-葡糖苷酶抑制作用。对葡萄葡萄糖苷抑制研究进行C.Papaya叶的水，甲醇，乙酸乙酯和正己烷提取物，甲醇提取物是最有效的（IC_50 = 171.83±26.89μg/ ml）。因此，通过柱色谱法进一步分离甲醇提取物，其产生六个合并的级分（A-F）和级分（Fa），显示最低IC_50（78.62±25.23μg/ ml）。随后，对体内麦芽糖和蔗糖耐受试验进行了对FA的抑制性对大鼠麦芽糖和蔗糖耐受性有效的抑制作用。曲线下的计算区域（AUC_（0-120））进一步表明FA（200mg / kg BW）在麦芽酶上具有更好的抑制活性而不是蔗糖酶。 FA的GC-MS分析表明存在酚类，各种脂肪酸及其衍生物。该研究表明，C.Papaya的叶子提取物具有α-葡糖苷酶抑制作用，并且可以在缓解餐后高血糖血症中被剥削。

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