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首页> 外文期刊>Acta microbiologica et immunologica Hungarica: A quarterly of the Hungarian Academy of Sciences >Cell wall ultrastructure of flocculent and non-flocculent Schizosaccharomyces pombe strains. Effect of cell wall hydrolysing enzymes on flocculation and cell wall ultastructure.
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Cell wall ultrastructure of flocculent and non-flocculent Schizosaccharomyces pombe strains. Effect of cell wall hydrolysing enzymes on flocculation and cell wall ultastructure.

机译：絮状和非絮状粟酒裂殖酵母菌株的细胞壁超微结构。细胞壁水解酶对絮凝和细胞壁超结构的影响。

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摘要

Scanning and transmission electron microscopic studies revealed the presence of slime-like, amorphous material on the surface of Schizosaccahromyces pombe RIVE 4-2-1 cells, independently, whether they were in flocculated or in non-flocculated state. Close contact of the adjacent cells via the merging outermost cell wall layers was found, however, only in the case of floc formation, which was induced by cultivating the cells in the presence of 6% (v/v) ethanol. Irreversible loss of the flocculation ability of the cells by treatment with proteinases suggests that proteinaceous cell surface molecules as lectins contribute to the cell-to-cell interaction during flocculation. Both proteinase K and pronase treatments removed a distinct outer layer of the cell wall, which indicated that the protein moieties of the phosphogalactomannan outer surface layer has a crucial role in the maintenance of cell wall integrity. In the case of lysing enzyme treatment the removal of the outermost layer was also observed as the first step of the cell wall digestion, while driselase treatment resulted in almost complete digestion of the cell wall.
机译：扫描和透射电镜研究表明，粟酒裂殖酵母RIVE 4-2-1细胞的表面呈粘液状，无定形物质，无论它们是絮凝状态还是非絮凝状态。但是，仅在絮凝物形成的情况下才发现相邻细胞通过合并的最外层细胞壁层紧密接触，这是通过在6％（v / v）乙醇存在下培养细胞而诱导的。通过用蛋白酶处理使细胞的絮凝能力不可逆转地损失表明，作为凝集素的蛋白质细胞表面分子在絮凝过程中促进了细胞间的相互作用。蛋白酶K和链霉菌蛋白酶处理均去除了细胞壁的独特外层，这表明磷酸半乳甘露聚糖外表面层的蛋白质部分在维持细胞壁完整性方面具有至关重要的作用。在裂解酶处理的情况下，也观察到最外层的去除是细胞壁消化的第一步，而干酶处理则导致细胞壁几乎完全消化。

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