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首页> 外文期刊>Acta microbiologica et immunologica Hungarica: A quarterly of the Hungarian Academy of Sciences >Colony morphology mutants of Trichoderma harzianum with increased beta-1,4-N-acetyl-glucosaminidase production.
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Colony morphology mutants of Trichoderma harzianum with increased beta-1,4-N-acetyl-glucosaminidase production.

机译：哈茨木霉菌的菌落形态突变体，其β-1,4-N-乙酰氨基葡萄糖苷酶产量增加。

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摘要

A total of 36 UV-induced mutants with altered colony morphology were isolated from strain Trichoderma harzianum T334, a potential biocontrol agent against plant pathogenic fungi with the ability to produce constitutively low levels of chitinases. The level of constitutive beta-1,4-N-acetyl-glucosaminidase production in standing and shaken cultures under non-inductive conditions was tested in mutants and compared to that of the parental strain. About 30% of the mutants showed significantly increased levels of enzyme production, with strain T334 col26a being the best producer. This mutant and the parental strain were subjected to in vitro confrontation assays with plant pathogenic Fusarium culmorum, Pythium debaryanum and Rhizoctonia solani strains. The mutant derivative could be characterized by significantly higher biocontrol index values than the parental strain in each experiment, suggesting, that mutants with improved constitutive extracellular chitinase secretion could be applied for biocontrol purposes against fungal plant pathogens.
机译：从哈氏木霉T334菌株中分离出总共36个紫外线诱导的具有改变的菌落形态的突变体，哈氏木霉T334是一种潜在的针对植物病原性真菌的生防剂，能够产生低水平的几丁质酶。在突变体中测试了在非诱导条件下静置和摇动培养物中组成型β-1,4-N-乙酰基-氨基葡糖苷酶的生产水平，并与亲本菌株进行了比较。大约30％的突变体显示出酶生产水平显着提高，其中菌株T334 col26a是最佳生产者。将该突变体和亲本菌株与植物病原性镰刀菌，腐霉腐霉和茄根枯菌进行体外对抗试验。在每个实验中，突变体衍生物的生物防治指数值均明显高于亲本菌株，这表明具有改善的组成型胞外几丁质酶分泌的突变体可用于针对真菌植物病原体的生物防治。

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