Characterization and heterologous expression of an age-dependent fungal/bacterial type chitinase of Aspergillus nidulans.

Erdei E; Pusztahelyi T; Miskei M; Barna T; Pocsi I

首页> 外文期刊>Acta microbiologica et immunologica Hungarica: A quarterly of the Hungarian Academy of Sciences >Characterization and heterologous expression of an age-dependent fungal/bacterial type chitinase of Aspergillus nidulans.

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Characterization and heterologous expression of an age-dependent fungal/bacterial type chitinase of Aspergillus nidulans.

机译：构巢曲霉的年龄依赖性真菌/细菌型几丁质酶的表征和异源表达。

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摘要

Under carbon starvation, Aspergillus nidulans produced a fungal/bacterial type chitinase, ChiB. The chiB gene was cloned and subcloned into pJC40 expression vector containing a 10XHis fusion tag, and the ChiB protein was expressed heterologously in Escherichia coli. Recombinant and native ChiB enzymes shared the same optimal pH ranges and showed similar substrate specificities with endo-acting cleavage patterns.

机译：在碳饥饿下，构巢曲霉产生了真菌/细菌型几丁质酶ChiB。将chiB基因克隆并亚克隆到含有10XHis融合标签的pJC40表达载体中，并且ChiB蛋白在大肠杆菌中异源表达。重组和天然ChiB酶具有相同的最佳pH范围，并显示相似的底物特异性和内切酶裂解模式。

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《Acta microbiologica et immunologica Hungarica: A quarterly of the Hungarian Academy of Sciences》 |2008年第3期|共11页
作者
Erdei E; Pusztahelyi T; Miskei M; Barna T; Pocsi I;
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原文格式 PDF
正文语种 eng
中图分类医学微生物学（病原细菌学、病原微生物学）;医学免疫学;微生物学;
关键词
Aspergillus nidulans; Blotting; Western; Chitinase; Cloning; Molecular; Enzyme Stability; 曲霉; 构巢; 印迹法; 蛋白质; 壳多糖酶; 克隆; 分子; 酶稳定性; 大肠杆菌; 基因表达;

机译：Aspergillus nidulans;Blotting;Western;Chitinase;Cloning;Molecular;Enzyme Stability;曲霉;构巢;印迹法;蛋白质;壳多糖酶;克隆;分子;酶稳定性;大肠杆菌;基因表达;

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1. Characterization and heterologous expression of an age-dependent fungal/bacterial type chitinase of Aspergillus nidulans. [J] . Erdei E, Pusztahelyi T, Miskei M, Acta microbiologica et immunologica Hungarica: A quarterly of the Hungarian Academy of Sciences . 2008,第3期

机译：构巢曲霉的年龄依赖性真菌/细菌型几丁质酶的表征和异源表达。
2. Differential expression and extent of fungal/plant and fungal/bacterial chitinases of Aspergillus fumigatus. [J] . Taib M, Pinney JW, Westhead DR, Archives of microbiology . 2005,第1期

机译：烟曲霉的真菌/植物和真菌/细菌几丁质酶的差异表达和程度。
3. Heterologous expression and characterization of CpI, OcpA, and novel serine-type carboxypeptidase OcpB from Aspergillus oryzae [J] . Morita H., Okamoto A., Yamagata Y., Applied Microbiology and Biotechnology . 2010,第2期

机译：米曲霉CpI，OcpA和新型丝氨酸型羧肽酶OcpB的异源表达和表征
4. Heterologous Expression of Aspergillus niger β-D-Xylosidase (XlnD): Characterization on Lignocellulosic Substrates [C] . Michael J. Selig, Eric P. Knoshaug, Stephen R. Decker, Symposium on Biotechnology for Fuels and Chemicals . 2008

机译：黑曲霉β-D-木糖苷酶（XlnD）的异源表达：木质纤维素底物上的表征。
5. Identification and characterization of mutants affecting the expression of the stc gene cluster in Aspergillus nidulans. [D] . Butchko, Robert Andrew Edward. 1999

机译：鉴定和表征影响构巢曲霉中stc基因簇表达的突变体。
6. Heterologous expression and characterization of CpI OcpA and novel serine-type carboxypeptidase OcpB from Aspergillus oryzae [O] . Hiroto Morita, Ayako Okamoto, Yohei Yamagata, -1

机译：米曲霉CpIOcpA和新型丝氨酸型羧肽酶OcpB的异源表达和表征
7. Heterologous expression and characterization of CpI, OcpA, and novel serine-type carboxypeptidase OcpB from Aspergillus oryzae [O] . Morita, Hiroto, Okamoto, Ayako, Yamagata, Yohei, 2009

机译：米曲霉CpI，OcpA和新型丝氨酸型羧肽酶OcpB的异源表达和表征

Characterization and heterologous expression of an age-dependent fungal/bacterial type chitinase of Aspergillus nidulans.

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