• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta microbiologica et immunologica Hungarica: A quarterly of the Hungarian Academy of Sciences >In vitro activity of fluconazole and amphotericin B against Candida inconspicua clinical isolates as determined by the time-kill method.
【24h】

In vitro activity of fluconazole and amphotericin B against Candida inconspicua clinical isolates as determined by the time-kill method.

机译：用时间杀灭法测定氟康唑和两性霉素B对抗隐性念珠菌临床分离株的体外活性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Candida inconspicua is an emerging pathogen in immunocompromised patients possessing inherently decreased susceptibility to fluconazole. We determined the MICs and killing activity of fluconazole and amphotericin B against C. inconspicua clinical isolates as well as reference strain C. inconspicua ATCC 16783 for comparison. MICs were determined using the standard broth microdilution method. Killing rates were determined using time-kill methodology at 0.5-16 x MIC fluconazole and amphotericin B concentrations. Fluconazole and amphotericin B MIC values varied between 16-128 mg/l and 0.5-1 mg/l, respectively. In time kill-assays fluconazole showed fungistatic effect at 1-16 x MIC concentrations against all tested strains after 24 h-incubation, but became fungicidal after 48 h at 4-16 x MIC concentrations. The time necessary to achieve fungicidal endpoint at 1 mg/l amphotericin B concentration ranged from 2 to 24 h. Our in vitro results confirm the data that fluconazole is ineffective against C. inconspicua at the fluconazole serum concentration attainable in humans. Amphotericin B due to its rapid killing activity seems to be a good alternative for the treatment of infections caused by C. inconspicua.
机译：隐性假丝酵母是免疫功能低下患者中的一种新兴病原体，其对氟康唑的易感性固有降低。我们确定了氟康唑和两性霉素B对隐球菌临床分离株以及参照菌株隐球菌ATCC 16783的MIC和杀伤活性，以进行比较。 MIC使用标准肉汤微量稀释法测定。使用杀灭方法在0.5-16 x MIC氟康唑和两性霉素B浓度下确定杀灭率。氟康唑和两性霉素B MIC值分别在16-128 mg / l和0.5-1 mg / l之间变化。随着时间的推移，在24小时孵育后，氟康唑对所有受试菌株在1-16 x MIC浓度下均表现出抑菌作用，但在4-16 x MIC浓度下48 h后对所有测试菌株均具有杀菌作用。两性霉素B浓度为1 mg / l达到杀菌终点所需的时间为2至24小时。我们的体外结果证实了以下数据：在人类可获得的氟康唑血清浓度下，氟康唑对不起的梭状芽胞杆菌无效。两性霉素B由于其快速的杀灭活性，似乎是治疗由隐球菌引起的感染的好选择。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号