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首页> 外文期刊>Crop & Pasture Science >Development of near-isogenic lines targeting a major QTL on 3AL for pre-harvest sprouting resistance in bread wheat
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Development of near-isogenic lines targeting a major QTL on 3AL for pre-harvest sprouting resistance in bread wheat

机译：在3AL上瞄准近代QTL的近代型线，在面包小麦中进行预先收获萌芽抗性

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摘要

Resistance to pre-harvest sprouting (PHS) in wheat (Triticum aestivum L.) is one of the most valuable traits in many breeding programs. However, the quantitative nature of inheritance of PHS resistance challenges the study of this trait. Near-isogenic lines (NILs) can turn a complicated quantitative trait into a Mendelian factor (qualitative) and are, therefore, valuable materials for identification of the gene(s) responsible for a specific phenotypic trait and for functional studies of specific loci. Five pairs of NILs were developed and confirmed for a major quantitative trait locus (QTL) located on the long arm of chromosome 3A contributing to PHS resistance in wheat. These NILs were generated by using the heterogeneous inbred family method and a fast generation-cycling system. Significant differences in PHS resistance between the isolines were detected in the NILs. The presence of the PHS-resistance allele from the resistant parent increased resistance to sprouting on spikes by 26.7-96.8%, with an average of 73.8%, and increased seed dormancy by 36.9-87.2%, with an average of 59.9% across the NILs. These NILs are being used for the identification of candidate genes responsible for this major PHS-resistance locus on wheat chromosome arm 3AL.
机译：小麦（Triticum aestivum L.）中收获前萌发（pH）的抗性是许多繁殖计划中最有价值的性状之一。然而，PHS抗性遗传的定量性质挑战这种特征的研究。近代线（NIL）可以将复杂的定量性状转变为孟德尔因子（定性），因此是有价值的材料，用于鉴定对特定表型性状的基因和对特定基因座的功能研究负责的基因。开发了五对尼尔，并确认位于染色体3A的长臂上的主要定量性状基因座（QTL）有助于小麦的pHS抗性。通过使用异质近交家庭方法和快速生成循环系统产生这些含量。在NIL中检测到分离物之间的pHS抗性的显着差异。来自耐药父母的pHS抗性等位基因的存在增加了26.7-96.8％，平均增加了26.7-96.8％，并增加了种子休眠36.9-87.2％，平均含有59.9％ 。这些含量用于鉴定对小麦染色体臂3Al上该主要pHS抗性基因座负责的候选基因。

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