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首页> 外文期刊>Cryobiology: International Journal of Low Temperature Biology and Medicine >Negative air pressure treatment accelerates the penetration of permeable cryoprotectants into bovine ovarian tissue in vitrification protocol and improves cell density after vitrification
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Negative air pressure treatment accelerates the penetration of permeable cryoprotectants into bovine ovarian tissue in vitrification protocol and improves cell density after vitrification

机译：负压处理加速可渗透的冷冻保护剂渗透到玻璃化方案中牛卵巢组织，并在玻璃化后提高细胞密度

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摘要

Effects of additional physical treatments during vitrification of the bovine ovarian tissue were examined for increasing of permeability of ethylene glycol (EG) and dimethyl sulfoxide (Me2SO). The concentrations of EG and Me2SO and histological changes in the ovarian tissue were evaluated. In the first equilibration step (7.5% EG and 7.5% Me2SO), all the 10-min physical treatments, i.e., negative (679 hPa) or positive (1347 hPa) air pressure applied with a disposable syringe, and shaking (60 rpm) applied with a laboratory shaker, were comparable to 25-min non-physical treatment (plain) vitrification. When effects of the negative air pressure were examined in the second equilibration step (20% EG and 20% Me2SO), its 10-min treatment was equivalent to 15-min plain vitrification (140-170 mg/g tissue). It was thus indicated that the negative air pressure treatment accelerates the penetration of permeable cryoprotectants into the ovarian tissue slices. Histological examination showed that the cell density and the amount of pan-cadherin in the tunica albuginea of the ovary was reduced by the vitrification, but was improved by the negative air pressure treatment. The amount of pan-cadherin in the tunica albuginea was recommended as a biomarker for evaluation of effectiveness of protocol for cryopreservation of bovine ovarian tissue and considered to be a candidate biomarker for human ovarian tissue cryopreservation.
机译：检查额外物理治疗在牛卵巢组织玻璃中的影响，用于增加乙二醇（例如）和二甲基亚砜（ME2SO）的渗透性。评估例如卵巢组织中的EG和ME2SO和组织学变化的浓度。在第一种平衡步骤（7.5％（例如，7.5％Me2SO）中，所有10分钟的物理处理，即负（679HPa）或阳性（1347 HPA）的空气压力施用一次性注射器，并摇动（60rpm）用实验室振荡器应用，与25分钟的非物理治疗（平原）玻璃化相当。当在第二平衡步骤（20％EG和20％ME2SO）中检查负空气压力的影响时，其10分钟的处理相当于15分钟的玻璃化（140-170mg / g组织）。因此表明，负压处理将可渗透的冷冻保护剂的渗透率加速到卵巢组织切片中。组织学检查表明，通过玻璃化降低了卵巢的卵巢中白杨中的细胞密度和泛钙蛋白的量，但通过负压处理得到改善。建议将泛钙蛋白的泛钙蛋白作为生物标志物，用于评估牛卵巢组织的冷冻保存方案的有效性，并被认为是人卵巢组织冷冻保存的候选生物标志物。

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