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首页> 外文期刊>Acta Biochimica Polonica >The effect of differentiation agents on inflammatory and oxidative responses of the human neuroblastoma cell line SK-N-SH
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The effect of differentiation agents on inflammatory and oxidative responses of the human neuroblastoma cell line SK-N-SH

机译：分化剂对人神经母细胞瘤细胞SK-N-SH炎症和氧化反应的影响

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摘要

Obtaining a suitable experimental cellular model is a major problem for neuroscience studies. Neuroblastoma cell lines have been often applied in studies related to pathological disorders of nervous system. However, in the search for an ideal model, these cells must be differentiated to cancel their tumor character. The subsequent reactions that are caused by differentiation are not always indifferent to the same model. We evaluated the effect of two well known substances, used for SH-N-SK cell line differentiation, retinoic acid (RA) and phorbol-12-myristate-13-acetate (PMA), on the induction of pro-inflammatory and pro-oxidative reactions in these cells. Cells differentiated with PMA were able to produce significantly higher amounts of pro-inflammatory cytokines whereas the release of nitric oxide radicals was similar to that in undifferentiated cells. On the contrary, in RA-differentiated cells no significant changes in cytokine production were observed and the nitric oxide release was decreased. Additionally, the RA-differentiated neuronal model was more sensible to lipopolysaccharide stimulation, producing pro-inflammatory cytokines abundantly. These results suggest that RA-differentiated SH-N-SK cells provide a more suitable experimental model for the study of molecular and cellular mechanisms of the inflammation and oxidative stress in neuronal cells.
机译：获得合适的实验性细胞模型是神经科学研究的主要问题。神经母细胞瘤细胞系通常被用于与神经系统病理疾病有关的研究中。然而，在寻找理想模型时，必须分化这些细胞以消除其肿瘤特征。由差异引起的后续反应并不总是对同一模型无动于衷。我们评估了用于SH-N-SK细胞分化的两种众所周知的物质，视黄酸（RA）和phorbol-12-肉豆蔻酸酯13-乙酸酯（PMA），对促炎和促炎的诱导作用。这些细胞中的氧化反应。用PMA分化的细胞能够产生大量的促炎细胞因子，而一氧化氮自由基的释放与未分化的细胞相似。相反，在RA分化的细胞中，未观察到细胞因子产生的显着变化，并且一氧化氮的释放减少。此外，RA分化的神经元模型对脂多糖刺激更敏感，从而大量产生促炎性细胞因子。这些结果表明，RA分化的SH-N-SK细胞为研究神经元细胞中炎症和氧化应激的分子和细胞机制提供了更合适的实验模型。

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