Application of the High Resolution Melting analysis for genetic mapping of Sequence Tagged Site markers in narrow-leafed lupin (Lupinus angustifolius L.)

Kamel Katarzyna A.; Kroc Magdalena; Swiecicki Wojciech

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Application of the High Resolution Melting analysis for genetic mapping of Sequence Tagged Site markers in narrow-leafed lupin (Lupinus angustifolius L.)

机译：高分辨率熔解分析在窄叶羽扇豆羽扇豆（Lupinus angustifolius L.）的序列标记位点标记的遗传作图中的应用

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摘要

Sequence tagged site (STS) markers are valuable tools for genetic and physical mapping that can be successfully used in comparative analyses among related species. Current challenges for molecular markers genotyping in plants include the lack of fast, sensitive and inexpensive methods suitable for sequence variant detection. In contrast, high resolution melting (HRM) is a simple and high-throughput assay, which has been widely applied in sequence polymorphism identification as well as in the studies of genetic variability and genotyping. The present study is the first attempt to use the HRM analysis to genotype STS markers in narrow-leafed lupin (Lupinus angustifolius L.). The sensitivity and utility of this method was confirmed by the sequence polymorphism detection based on melting curve profiles in the parental genotypes and progeny of the narrow-leafed lupin mapping population. Application of different approaches, including amplicon size and a simulated heterozygote analysis, has allowed for successful genetic mapping of 16 new STS markers in the narrow-leafed lupin genome.

机译：序列标记位点（STS）标记是用于遗传和物理作图的有价值的工具，可以成功地用于相关物种之间的比较分析中。植物中分子标记基因分型的当前挑战包括缺乏适用于序列变异检测的快速，灵敏和廉价的方法。相反，高分辨率熔解（HRM）是一种简单且高通量的测定方法，已广泛应用于序列多态性鉴定以及遗传变异性和基因分型研究中。本研究是首次尝试将HRM分析用于窄叶羽扇豆（Lupinus angustifolius L.）的STS标记基因型。通过基于窄叶羽扇豆作图群体的亲本基因型和后代中的熔解曲线谱的序列多态性检测，证实了该方法的敏感性和实用性。包括扩增子大小和模拟杂合子分析在内的不同方法的应用，已成功地在羽扇豆羽扇豆基因组中成功绘制了16种新的STS标记。

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《Acta Biochimica Polonica》 |2015年第3期|共8页
作者
Kamel Katarzyna A.; Kroc Magdalena; Swiecicki Wojciech;
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正文语种 eng
中图分类生物化学;
关键词
HRM; molecular markers; genotyping; narrow-leafed lupin;

机译：人力资源管理;分子标记;基因分型;羽扇豆羽扇豆;

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1. Application of the High Resolution Melting analysis for genetic mapping of Sequence Tagged Site markers in narrow-leafed lupin (Lupinus angustifolius L.) [J] . Kamel Katarzyna A., Kroc Magdalena, Swiecicki Wojciech Acta Biochimica Polonica . 2015,第3期

机译：高分辨率熔解分析在窄叶羽扇豆羽扇豆（Lupinus angustifolius L.）的序列标记位点标记的遗传作图中的应用
2. Development of sequence-specific PCR markers linked to the Tardus gene that reduces pod shattering in narrow-leafed lupin (Lupinus angustifolius L.) [J] . Jeffrey G. Boersma, Matthew N. Nelson, Krishnapillai Sivasithamparam, Molecular Breeding . 2009,第2期

机译：与Tardus基因连锁的序列特异性PCR标记的开发，可减少窄叶羽扇豆（Lupinus angustifolius L.）的荚果破碎
3. Development of sequence-specific PCR markers linked to the Tardus gene that reduces pod shattering in narrow-leafed lupin (Lupinus angustifolius L.) [J] . Boersma Jeffrey G., Nelson Matthew N., Sivasithamparam Krishnapillai, Molecular Breeding . 2009,第2期

机译：与Tardus基因连锁的序列特异性PCR标记的开发，可减少窄叶羽扇豆（Lupinus angustifolius L.）的荚果破碎
4. Effect of irrigation on growth and yield of Kabuli chickpea (Cicer arietinum L.) and narrow-leafed lupin (Lupinus angustifolius L.) [C] . S. Kang, B. A. McKenzie, G. D. Hill Annual Conference of the Agronomy Society of New Zealand . 2007

机译：灌溉对Kabuli chickpea（Cicer Arietinum L.）和窄羽磺的生长和产量的影响（Lupinus angustifolius L.）
5. The use of cpDNA SSR markers and sequenced fragments for comparative analysis of genetic relationships within the Cucurbitaceae and the inheritance of chilling injury in cucumber (Cucumis sativus L.) [D] . Chung, Sang-Min 2002

机译：使用cpDNA SSR标记和测序片段比较分析葫芦科内部的遗传关系和黄瓜冷害的遗传
6. Development and Validation of a Gene-Targeted dCAPS Marker for Marker-Assisted Selection of Low-Alkaloid Content in Seeds of Narrow-Leafed Lupin (Lupinus angustifolius L.) [O] . Magdalena Kroc, Katarzyna Czepiel, Paulina Wilczura, 2019

机译：开发和验证基因导向的dCAPS标记用于标记辅助选择窄叶羽扇豆羽扇豆（Lupinus angustifolius L.）种子中低生物碱含量
7. Development of two sequence-specific PCR markers linked to the le gene that reduces pod shattering in narrow-leafed Lupin (Lupinus angustifolius L.) [O] . Jeffrey G. Boersma, Bevan J. Buirchell, Krishnapillai Sivasithamparam, 2007

机译：开发与le基因连锁的两个序列特异性pCR标记，以减少窄叶羽扇豆（Lupinus angustifolius L.）中的豆荚破碎

Application of the High Resolution Melting analysis for genetic mapping of Sequence Tagged Site markers in narrow-leafed lupin (Lupinus angustifolius L.)

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