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Synthetic and genetically encoded fluorescent neural activity indicators

机译:合成和遗传编码的荧光神经活性指标

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摘要

The ultimate goal of neuroscience is to relate the complex activity of cells and cell-networks to behavior and cognition. This requires tools and techniques to visualize neuronal activity. Fluorescence microscopy is an ideal tool to measure activity of cells in the brain due to the high sensitivity of the technique and the growing portfolio of optical hardware and fluorescent sensors. Here, we give a chemist's perspective on the recent progress of fluorescent activity indicators that enable the measurement of cellular events in the living brain. We discuss advances in both chemical and genetically encoded sensors and look forward to hybrid indicators, which incorporate synthetic organic dyes into genetically encoded protein constructs.
机译:神经科学的最终目标是将细胞和细胞网络的复杂活性与行为和认知相关。 这需要工具和技术来可视化神经元活动。 由于技术的高灵敏度和光学硬件和荧光传感器的生长组合,荧光显微镜是测量脑中细胞活性的理想工具。 在这里,我们在最近的荧光活动指标进展方面提供了化学家的观点,这使得活性大脑中的细胞事件的测量。 我们讨论化学和遗传编码的传感器的进展,并期待杂交指示剂,其将合成有机染料掺入遗传编码的蛋白质构建体中。

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