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首页> 外文期刊>Current Microbiology: An International Journal >Comparative Analysis of Draft Genome Sequence of Rhodococcus sp. Eu-32 with Other Rhodococcus Species for Its Taxonomic Status and Sulfur Metabolism Potential
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Comparative Analysis of Draft Genome Sequence of Rhodococcus sp. Eu-32 with Other Rhodococcus Species for Its Taxonomic Status and Sulfur Metabolism Potential

机译：rhodococcus SP草案基因组序列的比较分析。 EU-32与其他菱康卡斯物种的分类物理和硫代谢潜力

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摘要

Rhodococcus sp. Eu-32 has shown an extended novel dibenzothiophene desulfurization sulfur-specific 4S pathway and could remove significant amounts of organic sulfur from coal. Here, we present the draft genome sequence of Eu-32 with a genome size of approximately 5.61 Mb, containing 5065 protein coding sequences with a G+C content of 65.1%. The Rhodococcus sp. Eu-32 showed similar to 99% identity at the 16S rRNA gene sequence level while < 34% digital DNA-DNA hybridization and < 81% average nucleotide identity values with the genome sequence of most closely related known Rhodococcus species, suggesting that it is taxonomically different from the already reported Rhodococcus species. Among the annotated genes, 90 are involved in the metabolism of sulfur. Comparative genome analysis suggests many commonalities in sulfur metabolism gene sets that may have evolved due to many factors including ecological pressures. Our study and the genome sequence data will be available for further research and will provide insights into potential biotechnological and industrial applications of this bacterium.
机译：rhodococcus sp。 Eu-32显示了一种延长的新型二苯甲酸噻吩脱硫硫特异性4S途径，可去除煤中大量的有机硫。这里，我们介绍了Eu-32的基因组序列草案，基因组大小约为5.61 MB，含有5065个蛋白质编码序列，G + C含量为65.1％。 rhodococcus sp。 Eu-32在16S rRNA基因序列水平上显示出99％的同一性，而使用最密切相关的rhodococcus种类的基因组序列<34％的数字DNA-DNA杂交和<81％的平均核苷酸同一性值，这表明它是分类的与已经报告的rhodococcus种类不同。在注释的基因中，90涉及硫的代谢。比较基因组分析表明，由于包括生态压力的许多因素，可能已经进化的硫代谢基因集中的许多共性。我们的研究和基因组序列数据将获得进一步的研究，并将提供对该细菌的潜在生物技术和工业应用的见解。

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    Akhtar Nasrin; Ghauri Muhammad A.; Akhtar Kalsoom; Parveen Sana; Farooq Muhammad; Ali Aamir; Schierack Peter;

  • 作者单位

    Natl Inst Biotechnol &

    Genet Engn Ind Biotechnol Div POB 577 Jhang Rd Faisalabad Pakistan;

    Natl Inst Biotechnol &

    Genet Engn Ind Biotechnol Div POB 577 Jhang Rd Faisalabad Pakistan;

    Natl Inst Biotechnol &

    Genet Engn Ind Biotechnol Div POB 577 Jhang Rd Faisalabad Pakistan;

    Natl Inst Biotechnol &

    Genet Engn Ind Biotechnol Div POB 577 Jhang Rd Faisalabad Pakistan;

    Natl Inst Biotechnol &

    Genet Engn Agr Biotechnol Div POB 577 Jhang Rd Faisalabad Pakistan;

    Natl Inst Biotechnol &

    Genet Engn Hlth Biotechnol Div POB 577 Jhang Rd Faisalabad Pakistan;

    Brandenburg Univ Technol Cottbus Senftenberg Inst Biotechnol Senftenberg Germany;

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  • 正文语种 eng
  • 中图分类 微生物学;
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