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Good Citizenship Made Easy: AStep-by-Step Guide to SubmittingRNA-Seq Data to NCBI

机译:良好的公民身份简单:将ASTEPTEPTRNA-SEQ数据指南逐步指南到NCBI

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摘要

The analysis of transcriptome data from non-model organisms contributes toour understanding of diverse aspects of evolutionary biology, including developmentalprocesses, speciation, adaptation, and extinction. Underlying this diversityis one shared feature, the generation of enormous amounts of sequencedata. Data availability requirements in most journals oblige researchers tomake their raw transcriptome data publicly available, and the databases housedat the National Center for Biotechnology Information (NCBI) are a popularchoice for data deposition. Unfortunately, the successful submission of rawsequences to the Sequence Read Archive (SRA) and transcriptome assembliesto the Transcriptome Shotgun Assembly (TSA) can be challenging for noviceusers, significantly delaying data availability and publication. Here we presenttwo comprehensive protocols for submitting RNA-Seq data to NCBI databases,accompanied by an easy-to-use website that facilitates the timely submissionof data by researchers of any experience level.
机译:来自非模型生物的转录组数据分析有助于了解进化生物学的各种方面,包括发展过程,品质,适应和灭绝。这种多样性的底层共享特征,产生大量的Sequenceata。数据可用性要求在大多数期刊上要求研究人员在公开可用的原始转录组数据中割伤,而数据库则拥有国家生物技术信息中心(NCBI)是数据沉积的普令妙。遗憾的是,成功将Rawsequences成功提交到序列读取档案(SRA)和转录组合汇编程序的转录组霰弹枪组件(TSA)可能对NoviceUsers有挑战性,显着延迟数据可用性和出版物。在这里,我们介绍了将RNA-SEQ数据提交给NCBI数据库的全面协议,并附有一个易于使用的网站,促进任何经验级别的研究人员及时提交数据。

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