An On-Chip Method for Long-TermGrowth and Real-Time Imaging of Brain

Organoids Eyal Karzbrun; Rami Yair Tshuva; Orly Reiner

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An On-Chip Method for Long-TermGrowth and Real-Time Imaging of Brain

机译：一种用于长期血统和大脑实时成像的片上方法

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Brain organoids are an emerging technique for studying human neurodevelopmentin vitro, with biomedical implications. However, three-dimensionaltissue culture poses several challenges, including lack of nutrient exchange atthe organoid core and limited imaging accessibility of whole organoids. Herewe present a method for culturing organoids in a micro-fabricated device thatenables in situ real-time imaging over weeks with efficient nutrient exchangeby diffusion. Our on-chip approach offers a means for studying the dynamicsof organoid development, cell differentiation, cell cycle, and motion.

机译：脑器有机体是一种用于学习人类神经发作的体外的新兴技术，具有生物医学意义。然而，三维疾病培养造成几种挑战，包括缺乏有机体核心的营养交换和整个有机体的有限的成像可用性。这里介绍一种在微制造的装置中培养有机体的方法，尤其是具有高效营养的非扩散的数周内原位实时成像。我们的片上方法提供了用于研究有机体开发，细胞分化，细胞周期和运动的动态的方法。

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来源
《Current Protocols in Cell Biology》 |2018年第1期|共17页
作者
Organoids Eyal Karzbrun; Rami Yair Tshuva; Orly Reiner;
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作者单位

Department of Molecular Genetics Weizmann Institute of Science Rehovot Israel;

Department of Physics and the Kavli Institute for Theoretical Physics University ofCalifornia Santa Barbara Santa Barbara California;

Department of Molecular Genetics Weizmann Institute of Science Rehovot Israel;

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原文格式 PDF
正文语种 eng
中图分类细胞生物学;
关键词
brain organoids; human pluripotent stem cells; lab on a chip; microfabrication; 3D cell culture; neurodevelopment;

机译：脑器有机体;人类多能干细胞;芯片上的实验室;微制造;3D细胞培养;神经发育;

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1. An On-Chip Method for Long-TermGrowth and Real-Time Imaging of Brain [J] . Organoids Eyal Karzbrun, Rami Yair Tshuva, Orly Reiner Current Protocols in Cell Biology . 2018,第1期

机译：一种用于长期血统和大脑实时成像的片上方法
2. Fully On-Chip Integrated Photodetector Front-End Dedicated to Real-Time Portable Optical Brain Imaging [J] . Ehsan Kamrani, Frederic Lesage, Mohamad Sawan Optics and Photonics Journal . 2012,第4期

机译：专用于实时便携式光学脑成像的全片上集成光电探测器前端
3. An on-chip imaging droplet-sorting system: a real-time shape recognition method to screen target cells in droplets with single cell resolution [J] . Mathias Girault, Hyonchol Kim, Hisayuki Arakawa, Scientific reports. . 2017,第1期

机译：片上成像液滴排序系统：具有单个小区分辨率的液滴中屏幕目标单元的实时形状识别方法
4. On-Chip Single Photon Counting Electronic Circuitry Dedicated to Real-Time Brain Imaging Applications [C] . Kamrani Ehsan, Lesage Frederic, Sawan Mohamad Southern Biomedical Engineering Conference . 2013

机译：专用于实时脑成像应用的片上单光子计数电子电路
5. On-chip integrated functional near infra-red spectroscopy (fNIRS) photoreceiver for portable brain imaging. [D] . Kamrani, Ehsan. 2014

机译：片上集成功能性近红外光谱（fNIRS）光接收器，用于便携式大脑成像。
6. An on-chip imaging droplet-sorting system: a real-time shape recognition method to screen target cells in droplets with single cell resolution [O] . Mathias Girault, Hyonchol Kim, Hisayuki Arakawa, -1

机译：片上成像液滴分选系统：实时形状识别方法以单细胞分辨率筛选液滴中的靶细胞
7. Fully On-Chip Integrated Photodetector Front-End Dedicated to Real-Time Portable Optical Brain Imaging [O] . 2012

机译：专用于实时便携式光学脑成像的全片上集成光电探测器前端

An On-Chip Method for Long-TermGrowth and Real-Time Imaging of Brain

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