Determination of Membrane ProteinDistribution on the Nuclear Envelope bySingle-Point Single-Molecule FRAP

Krishna C. Mudumbi; Weidong Yang

首页> 外文期刊>Current Protocols in Cell Biology >Determination of Membrane ProteinDistribution on the Nuclear Envelope bySingle-Point Single-Molecule FRAP

【24h】

Determination of Membrane ProteinDistribution on the Nuclear Envelope bySingle-Point Single-Molecule FRAP

机译：核包封膜蛋白分布的测定通过铰接点单分子FRAP

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Nuclear envelope transmembrane proteins (NETs) are synthesized on the endoplasmicreticulum and then transported from the outer nuclear membrane(ONM) to the inner nuclear membrane (INM) in eukaryotic cells. The abnormaldistribution of NETs has been associated with many human diseases.However, quantitative determination of the spatial distribution and translocationdynamics of NETs on the ONM and INM is still very limited in currentlyexisting approaches. Here we demonstrate a single-point single-molecule fluorescencerecovery after photobleaching (FRAP) microscopy technique thatenables quick determination of distribution and translocation rates for NETsin vivo. C 2017 by John Wiley & Sons, Inc.

机译：核包膜跨膜蛋白蛋白（网）在内皮内核上合成，然后从外核膜（ONM）输送到真核细胞中的内核膜（INM）。网的异常分布与许多人类疾病有关。但是，在ONM和INM上的空间分布和互联网上的空间分布和易位动力学的定量确定仍然非常有限。在这里，我们在光漂白剂（FRAP）显微镜技术后表现出单点单分子荧光素再生，鉴于Netsin体内的分布和易位率的快速测定。 C 2017由John Wiley＆Sons，Inc。

著录项

来源
《Current Protocols in Cell Biology》 |2017年第76期|共21页
作者
Krishna C. Mudumbi; Weidong Yang;
展开▼
作者单位

Department of Biology Temple University Philadelphia Pennsylvania;

Department of Biology Temple University Philadelphia Pennsylvania;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类细胞生物学;
关键词
Cellular imaging; membrane biophysics; super-resolutiontransmembrane protein; FRAP;

机译：细胞成像;膜生物物理学;超分辨率三颅蛋白;FRAP;

相似文献

外文文献
中文文献
专利

1. Determination of Membrane ProteinDistribution on the Nuclear Envelope bySingle-Point Single-Molecule FRAP [J] . Krishna C. Mudumbi, Weidong Yang Current Protocols in Cell Biology . 2017,第Suppla76期

机译：核包封膜蛋白分布的测定通过铰接点单分子FRAP
2. Determination of Membrane Protein UNIT 21.11Distribution on the Nuclear Envelope bySingle-Point Single-Molecule FRAP [J] . Krishna C. Mudumbi, Weidong Yang Current Protocols in Cell Biology . 2018,第1期

机译：膜蛋白单元21.11中的核围影型单分子FRAP的测定
3. Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution [J] . Krishna C Mudumbi, Eric C Schirmer, Weidong Yang Nature Communications . 2016,第1期

机译：单点单分子FRAP区分内外核膜蛋白分布
4. Single-molecule imaging, force measurement and fluorescence observation reveal protein and chromosome dynamics around the nuclear envelope [C] . Otsuka S., Hirano Y., Takahashi H., International Symposium on Micro-NanoMechatronics and Human Science . 2007

机译：单分子成像，力测量和荧光观察显示核信封周围的蛋白质和染色体动力学
5. Determining Features Sufficient for Protein Trafficking to the Plant Inner Nuclear Membrane and Identification of Putative Nuclear Envelope-associated Proteins in Arabidopsis thaliana [D] . Groves, Norman R. 2019

机译：确定足以用于植物内核膜的蛋白质的特征和拟定核包封相关蛋白质拟南芥拟拟合
6. Determination of Membrane Protein Distribution on the Nuclear Envelope by Single-Point Single-Molecule FRAP [O] . Krishna C. Mudumbi, Weidong Yang -1

机译：单点单分子FRAP法测定核膜上的膜蛋白分布
7. Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution [O] . Mudumbi, Krishna C., Schirmer, Eric C., Yang, Weidong 2016

机译：单点单分子FRap区分内核膜蛋白和外核膜蛋白分布

Determination of Membrane ProteinDistribution on the Nuclear Envelope bySingle-Point Single-Molecule FRAP

摘要

著录项

相似文献

相关主题

期刊订阅