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首页> 外文期刊>Current Protocols in Cell Biology >Generating Embryonic Salivary Gland Organoids
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Generating Embryonic Salivary Gland Organoids

机译：产生胚胎唾液腺有机体

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摘要

Organoids are important research tools for studying organ morphogenesis and differentiation because they recapitulate ex vivo the native 3D organization of cells that is essential for proper cell and organ function. The composition of organoids can bemanipulated to incorporate specific cell types to facilitate molecular interrogation of cell-cell interactions during organoid formation. A method for generating organoids derived from both embryonic salivary gland epithelial progenitor cells and mesenchymal support cells is described. Methods for isolating enriched populations of the epithelial cells as clusters and the mesenchyme cells as single cells from mouse embryonic submandibular salivary glands are also provided. Separating the epithelial andmesenchymal cell populations allows for independent molecular manipulation of each cell type. In addition, methods for lentiviral transduction of the mesenchyme cells and quantitative image analysis of organoids are provided. The methods described here are useful for exploring mechanisms driving organ formation.
机译：有机体是用于研究器官形态发生和分化的重要研究工具，因为它们概括了exvivo对适当细胞和器官功能至关重要的细胞的天然3D组织。有机体的组合物可以Bemanipulation以掺入特定细胞类型以促进细胞体形成期间细胞细胞相互作用的分子询问。描述了一种用于产生衍生自胚胎唾液腺上皮祖细胞和间充质支持细胞的有机体的方法。还提供了将富集的上皮细胞的富集群作为簇和间本细胞作为来自小鼠胚胎颌下涎腺唾液腺的单细胞分离的方法。分离上皮和发粒细胞群允许对每个细胞类型的独立分子操纵。另外，提供了间充质细胞的慢病毒转导的方法和有机体的数量图像分析。这里描述的方法可用于探索驱动器官形成的机制。

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