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首页> 外文期刊>Acta biomaterialia >Stability and bioactivity of pepCD47 attachment on stainless steel surfaces
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Stability and bioactivity of pepCD47 attachment on stainless steel surfaces

机译：Pepcd47附着在不锈钢表面上的稳定性和生物活性

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In-stent restenosis (ISR) and late stent thrombosis are the major complications associated with the use of metal stents and drug eluting stents respectively. Our lab previously investigated the use of peptide CD47 in improving biocompatibility of bare metal stents in a rat carotid stent model and our results demonstrated a significant reduction in platelet deposition and ISR. However, this study did not characterize the stability of the pepCD47 on metal surfaces post storage, sterilization and deployment. Thus, the objective of the present study was 1) to test the stability of the peptide post - storage, sterilization, exposure to shear and mechanical stress and 2) to begin to expand our current knowledge of pepCD47 coated metal surfaces into the preclinical large animal rabbit model. Our results show that the maximum immobilization density of pepCD47 on metal surfaces is approximately 350 ng/cm(2). 100% of the pepCD47 was retained on the metal surface post 24 weeks of storage at 4 degrees C, exposure to physiological shear stress, and mechanical stress of stent expansion. The bioactivity of the pepCD47 was found to be intact post 24 weeks of storage and ethylene oxide sterilization. Finally our ex vivo studies demonstrated that compared to bare metal the rabbit pepCD47 coated surfaces showed - 45% reduced platelet adhesion, a 10-fold decrease in platelet activation, and 93% endothelial cell retention. Thus, our data suggests that pepCD47 coating on metal surfaces is stable and rabbit pepCD47 shows promising preliminary results in preventing thrombosis and not inhibiting the growth of endothelial cells.
机译：支架再狭窄（ISR）和晚期支架血栓形成是与使用金属支架和药物洗脱支架相关的主要并发症。我们的实验室先前研究了肽CD47在提高大鼠颈动脉支架模型中改善裸金属支架的生物相容性，我们的结果表明血小板沉积和ISR的显着降低。然而，本研究没有表征Pepcd47对金属表面后储存，灭菌和部署的稳定性。因此，本研究的目的是1）以测试肽储存后的稳定性，灭菌，暴露于剪切和机械应力和2）开始扩大我们目前的Pepcd47涂层金属表面的知识进入临床前大型动物兔模型。我们的结果表明，金属表面上的Pepcd47的最大固定密度约为350ng / cm（2）。将100％的Pepcd47保留在4℃的24周内储存的金属表面柱上，暴露于生理剪切应力，以及支架膨胀的机械应力。发现Pepcd47的生物活性是完整的24周储存和环氧乙烷灭菌的术后。最后，我们的前体内研究表明，与裸晶金属相比，兔Pepcd47涂覆表面降低了血小板粘附减少，血小板激活减少10倍，内皮细胞保留量为93％。因此，我们的数据表明，金属表面上的Pepcd47涂层是稳定的，并且兔Pepcd47显示有前途的初步导致预防血栓形成并且不抑制内皮细胞的生长。

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