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首页> 外文期刊>Acta biomaterialia >Waveguiding and focusing in a bio-medium with an optofluidic cell chain
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Waveguiding and focusing in a bio-medium with an optofluidic cell chain

机译：波导和聚焦在具有邻接流体细胞链的生物培养基中

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摘要

Long-distance waveguiding and submicron focusing of light in a bio-medium are crucial for biomedical sensing and imaging. Disordered bio-mediums usually exhibit high scattering and absorption, which limits effective waveguiding and focusing. Here, we demonstrate an optofluidic cell chain, assembled via an optical trapping force from an optical fiber probe, to achieve long-distance waveguiding and submicron light focusing in a disordered bio-medium. By applying a trapping light at 980 nm to generate an optical force, stable binding of E. faecalis cells was achieved in a fluid to assemble cell chains of different lengths. The length could reach up to 360 mu m and the incident light (at 675, 532 and 473 nm) could be focused into a beam with a waist radius of 400 nm. As a potential practical application, backscattered signals from human red blood cells were detected using the cell chains, which is expected to benefit biomedical sensing and single cell analysis.
机译：生物介质中光的长距离波动和亚微米对生物医学传感和成像至关重要。 无序的生物介质通常具有高散射和吸收，这限制了有效的波动和聚焦。 这里，我们展示了通过来自光纤探针的光学俘获力组装的替代物流细胞链，以实现长距离波动和聚焦在无序的生物介质中的亚微米光。 通过在980nm处施加捕获光以产生光学力，在流体中达到粪便细胞的稳定结合以组装不同长度的细胞链。 长度可以达到高达360μm，并且入射光（在675,532和473nm处）可以聚焦到具有400nm的腰半径的梁中。 作为潜在的实际应用，使用细胞链检测来自人红细胞的反向散射信号，这预计将受益生物医学感测和单细胞分析。

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