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首页> 外文期刊>Acta biomaterialia >Assembly of erodible, DNA-containing thin films on the surfaces of polymer microparticles: toward a layer-by-layer approach to the delivery of DNA to antigen-presenting cells.
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Assembly of erodible, DNA-containing thin films on the surfaces of polymer microparticles: toward a layer-by-layer approach to the delivery of DNA to antigen-presenting cells.

机译：在聚合物微粒的表面上组装易于的DNA的薄膜：朝向逐层递送DNA以抗原呈递细胞。

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We report a layer-by-layer approach to the assembly of ultrathin and erodible DNA-containing films on the surfaces of polymer microparticles. DNA-containing multilayered films were fabricated layer-by-layer on the surfaces of polystyrene microspheres (approximately 6 microm) by iterative and alternating cycles of particle suspension, centrifugation and resuspension in solutions of plasmid DNA and a hydrolytically degradable polyamine. Film growth occurred in a stepwise manner, as demonstrated by characterization of the zeta potentials and fluorescence intensities of film-coated particles during film assembly. Characterization of film-coated particles by confocal fluorescence microscopy and scanning electron microscopy revealed the multilayered particle coatings to be smooth, uniform and free of large-scale physical defects. Film-coated microparticles sustained the release of transcriptionally active DNA into solution for approximately three days when incubated in physiologically relevant media. Previous studies have demonstrated that the adsorption of DNA onto the surfaces of cationic microparticles can be used to target the delivery of DNA to antigen-presenting cells. As a first step toward the application of this layer-by-layer approach to the development of methods for the delivery of DNA to antigen-presenting cells, we demonstrated that film-coated microparticles could be used to transport DNA into macrophage cells in vitro using a model mouse macrophage cell line. Our results suggest the basis of a general approach that could, with further development, prove useful for the delivery of DNA-encoded antigens to macrophages, or other antigen-presenting cells, and provide new materials-based methods for the formulation and delivery of DNA vaccines.
机译：我们在聚合物微粒的表面上报告了将逐层含有超薄和可蚀的DNA薄膜组装的方法。通过迭代和交替的颗粒悬浮液，离心和重悬浮在质粒DNA溶液和水解可降解的多胺的溶液中的逐层制造含DNA的多层膜在聚苯乙烯微球（约6μm）的表面上是制造的层的层上层。通过在薄膜组件期间表征膜涂覆颗粒的Zeta电位和荧光强度来表征逐步的方式发生膜生长。通过共聚焦荧光显微镜和扫描电子显微镜表征膜涂膜颗粒揭示了多层颗粒涂层光滑，均匀，没有大规模的物理缺陷。膜涂覆的微粒在在生理相关培养基中孵育时，将转录活性DNA的释放到溶液中约为三天。以前的研究表明，DNA在阳离子微粒的表面上吸附可用于靶向DNA至抗原呈递细胞的递送。作为朝向将该层逐层施加到抗原呈递细胞递送DNA的方法的第一步的第一步，我们证明膜涂覆的微粒可用于在体外将DNA传送到巨噬细胞中模型小鼠巨噬细胞细胞系。我们的研究结果表明，通过进一步发展，可以有助于将DNA编码的抗原递送给巨噬细胞或其他抗原呈递细胞，并提供新的基于材料的制剂和递送DNA的方法的基础疫苗。

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