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首页> 外文期刊>Acta biomaterialia >Red fluorescent AuNDs with conjugation of cholera toxin subunit B (CTB) for extended-distance retro-nerve transporting and long-time neural tracing
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Red fluorescent AuNDs with conjugation of cholera toxin subunit B (CTB) for extended-distance retro-nerve transporting and long-time neural tracing

机译：红荧光白光白炽随着霍乱毒素亚基（CTB）的缀合，用于延长距离复古 - 神经运输和长期神经追踪

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摘要

A retrograde transportation nerve probe, Au nanodots-cholera toxin B subunit (AuNDs-CTB), are prepared and fully characterized, which emit bright red fluorescence and show high quantum yield (7.2%) and good stability. The fluorescence emitted by the AuNDs is constant across a wide pH range (4-10) and after prolonged UV irradiation (>4 h). Previously, CTB has shown targeting characteristic for nerve cells with high sensitivity and effectiveness. After linking CTB to AuNDs through amidation reactions, AuNDs-CTB are obtained with excellent fluorescence property, nerve target characteristic, and, particularly, neural retrograde transportation feature. The red emission of the AuNDs-CTB is well distinguished from the blue autofluorescence of normal tissues, which provides potential for detection by naked eyes. Further, the fluorescence emission intensity maintains for 10 days in vivo, suggesting great utility for long-time monitoring and sensing of the nerve tissue. Furthermore, the AuNDs-CTB with bright red fluorescence can travel through the peripheral nerve to the spinal cord rapidly by retrograde transportation. The transportation occurs for a long distance (>5 cm) within only 2 days after injection of the AuNDs-CTB into the sciatic nerve. The present study exhibits a novel method for nerve visualization and drug delivery.
机译：制备逆行运输神经探针Au纳米蛋白 - 霍乱毒素B亚基（AUNDS-CTB），并完全表征，发出明亮的红色荧光并显示出高量子产率（7.2％）和良好的稳定性。 AUNDS发射的荧光在宽的pH范围（4-10）上恒定，延长紫外线照射（> 4小时）。以前，CTB显示了具有高灵敏度和有效性的神经细胞的靶向特征。通过酰胺化反应将CTB与AUNDS联系起来后，通过优异的荧光性，神经靶特性，特别是神经逆行运输特征获得AUNDS-CTB。 AUNDS-CTB的红色发射良好地与正常组织的蓝色自发荧光分开，这提供了通过肉眼检测的潜力。此外，荧光发射强度在体内维持10天，表明长期监测和感测神经组织的效用。此外，具有亮红色荧光的AUNDS-CTB可以通过逆行运输快速地通过周围神经到脊髓。在将AUNDS-CTB注入坐骨神经后，仅在2天内进行长距离（> 5cm）的运输。本研究表现出一种用于神经可视化和药物递送的新方法。

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  • 来源
    《Acta biomaterialia》 |2020年第2020期|共9页
  • 作者

    Zhao Yueqi; Maharjan Suraj; Sun Yuanqing; Yang Zhe; Yang Enfeng; Zhou Nan; Lu Laijin; Whittaker Andrew K.; Yang Bai; Lin Quan;

  • 作者单位

    Jilin Univ Coll Chem State Key Lab Supramol Struct &

    Mat Changchun 130012 Peoples R China;

    First Hosp Jilin Univ Jilin Prov Key Lab Tissue Repair Reconstruct &

    Re Dept Hand Surg Changchun;

    China Univ Petr Coll Sci State Key Lab Heavy Oil Proc Beijing 102249 Peoples R China;

    Jilin Univ Coll Chem State Key Lab Supramol Struct &

    Mat Changchun 130012 Peoples R China;

    Jilin Univ Coll Chem State Key Lab Supramol Struct &

    Mat Changchun 130012 Peoples R China;

    Zhengzhou Univ Affiliated Hosp 1 Dept Orthoped 1 Jianshe Rd Zhengzhou 450000 Peoples R China;

    First Hosp Jilin Univ Jilin Prov Key Lab Tissue Repair Reconstruct &

    Re Dept Hand Surg Changchun;

    Univ Queensland Australian Inst Bioengn &

    Nanotechnol Brisbane Qld 4072 Australia;

    Jilin Univ Coll Chem State Key Lab Supramol Struct &

    Mat Changchun 130012 Peoples R China;

    Jilin Univ Coll Chem State Key Lab Supramol Struct &

    Mat Changchun 130012 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 普通生物学;
  • 关键词

    Red fluorescent AuNDs-CTB; Nerve visualization; Neural rapid retro-transporting; Extended-distance transportation; Long-time neural trace;

    机译：红色荧光AUNDS-CTB;神经可视化;神经快速复古;延长距离运输;长期神经迹线;

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