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首页> 外文期刊>Acta biomaterialia >Co-immobilization of CD133 antibodies, vascular endothelial growth factors, and REDV peptide promotes capture, proliferation, and differentiation of endothelial progenitor cells
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Co-immobilization of CD133 antibodies, vascular endothelial growth factors, and REDV peptide promotes capture, proliferation, and differentiation of endothelial progenitor cells

机译：CD133抗体，血管内皮生长因子和RedV肽的共同固定促进内皮祖细胞的捕获，增殖和分化

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摘要

Capture of endothelial progenitor cells (EPCs) in situ has been considered as a promising strategy for the rapid endothelialization and long-term patency of artificial blood vessels and implant devices. In this study, a CD133(+) EPC capture surface was fabricated by grafting CD133 antibody (a more specific EPC surface marker than CD34) and Arg-Glu-Asp-Val (REDV) peptideon the methacrylate-grafted hyaluronic acid (MA-HA) and heparin-hybridized (MA-HA&Heparin) resisting layer. Vascular endothelial growth factor (VEGF) was further conjugated to the immobilized heparin. This engineered surface showed good hemocompatibility and significantly higher ability of capturing CD133(+) EPCs from human peripheral blood mononuclear cells (PBMCs) and obviously upregulated the expression of endothelial cell (EC) marker genes of EPCs such as VEGF receptor 2 (VEGFR2), CD31, VE-cadherin, and von Willebrand factor (vWF), facilitating the differentiation of EPCs into ECs. The dramatically enhanced EPC proliferation on this surface was dependent on the integrin-VEGFR synergistic signaling, as ERK1/2 phosphorylation was only significantly enhanced on the REDV and VEGF co-immobilized surface. This study highlights a new surface coating strategy for blood-contact materials based on the specific EPC capturing and rapid endothelialization.
机译：原位捕获内皮祖细胞（EPC）被认为是人造血管和植入装置的快速内皮化和长期通畅的有希望的策略。在该研究中，通过嫁接CD133抗体（比CD34更特异的EPC表面标记物）制备CD133（+）EPC捕获表面，并且甲基丙烯酸酯 - 移植的透明质酸（MA-HA） ）和肝素杂交（MA-HA＆肝素）抵抗层。血管内皮生长因子（VEGF）进一步与固定的肝素缀合。该工程表面呈现出良好的血液振动性，捕获来自人外周血单核细胞（PBMC）的CD133（+）EPC的能力明显更高，并明显上调EPC受体2（VEGFR2）如VEGF受体2（VEGFR2）的内皮细胞（EC）标志物基因的表达， CD31，Ve-Cadherin和von Willebrand因子（VWF），促进EPC分化为ECS。在该表面上显着增强的EPC增殖取决于整联素-Vemfr协同信号传导，因为ERK1 / 2磷酸化仅在REDV和VEGF共固定表面上显着增强。本研究突出了基于特定EPC捕获和快速内皮化的血液接触材料的新表面涂层策略。

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