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首页> 外文期刊>Acta biomaterialia >Photochemically crosslinked collagen annulus plug: A potential solution solving the leakage problem of cell-based therapies for disc degeneration
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Photochemically crosslinked collagen annulus plug: A potential solution solving the leakage problem of cell-based therapies for disc degeneration

机译：光化学交联的胶原环形塞：解决基于细胞疗法的漏电问题的潜在解决方法

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摘要

Intra-disc injection of mesenchymal stem cells (MSCs) to treat disc degeneration may lead to unfavorable complications, particularly osteophyte formation. Development of an effective method to block the injection portal, prevent the leakage of injected cells and materials and, hence, prevent osteophyte formation is of the utmost importance before MSC-based therapies can be applied in a clinical setting. Here we seek to alleviate the cell leakage problem and the associated complication osteophyte formation by developing an injectable annulus plug to block the injection portal during intra-disc delivery. Specifically, we fabricated a needle-shaped collagen plug by photochemical crosslinking and successfully delivered it intra-discally, in association with MSCs in collagen microsphere carriers, using a custom-made delivery device. The mechanical performance of the plug and its effectiveness in reducing cell leakage were evaluated ex vivo under compression and in torsion push-out tests. The results demonstrate that the plug survived physiologically relevant loadings and significantly reduced leakage and enhanced retention of the injected materials. Finally, a pilot in vivo study in rabbits was conducted to evaluate the performance of the plug. Microcomputed tomography imaging and histology revealed that the plug significantly reduced osteophyte formation. This work suggests the potential of the annulus plug as an adjunct or annulus closure device for intra-disc delivery of cells and materials.
机译：盘中注射间充质干细胞（MSCs）治疗盘变性可能导致不利的并发症，特别是骨赘形成。开发一种阻断注射门户的有效方法，防止注射细胞和材料的泄漏，因此，在基于MSC的疗法可以在临床环境中应用之前，预防骨赘形成是至关重要的。在这里，我们试图通过开发可注射的环形插头来缓解细胞泄漏问题和相关的并发症骨赘形成，以在盘内输送过程中阻挡注射门户。具体地，我们使用定制的输送装置，通过光化学交联通过光化学交联并成功地与MSCs致疑紧地递送它的针形胶原蛋白。在压缩和扭转推出试验下，评估了塞子的机械性能及其降低细胞泄漏的有效性。结果表明，塞子存活生理相关的载荷，并显着降低泄漏和增强的注入材料的保留。最后，进行了在兔子中的体内研究中的飞行员，以评估插头的性能。微型断层摄影成像和组织学揭示了插头显着降低了骨赘形成。这项工作表明环形插头作为用于盘内递送细胞和材料的辅助封闭装置的潜力。

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  • 来源
    《Acta biomaterialia》 |2013年第9期|共12页
  • 作者

    ChikT.K.; MaX.Y.; ChoyT.H.; LiY.Y.; DiaoH.J.; TengW.K.; HanS.J.; CheungK.M.C.; ChanB.P.;

  • 作者单位

    Tissue Engineering Laboratory Department of Mechanical Engineering University of Hong Kong;

    Tissue Engineering Laboratory Department of Mechanical Engineering University of Hong Kong;

    Tissue Engineering Laboratory Department of Mechanical Engineering University of Hong Kong;

    Tissue Engineering Laboratory Department of Mechanical Engineering University of Hong Kong;

    Tissue Engineering Laboratory Department of Mechanical Engineering University of Hong Kong;

    Tissue Engineering Laboratory Department of Mechanical Engineering University of Hong Kong;

    Tissue Engineering Laboratory Department of Mechanical Engineering University of Hong Kong;

    Department of Orthopaedics and Traumatology Li Ka Shing Faculty of Medicine University of Hong;

    Tissue Engineering Laboratory Department of Mechanical Engineering University of Hong Kong;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 普通生物学;
  • 关键词

    Annulus plug; Cell leakage; Intra-disc injection; Osteophyte formation; Photochemical crosslinking;

    机译：环形塞;细胞泄漏;盘中注射;骨赘形成;光化学交联;

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