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首页> 外文期刊>Acta biomaterialia >Tissue engineered hydrogels supporting 3D neural networks
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Tissue engineered hydrogels supporting 3D neural networks

机译：支持3D神经网络的组织工程水凝胶

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Promoting nerve regeneration requires engineering cellular carriers to physically and biochemically support neuronal growth into a long lasting functional tissue. This study systematically evaluated the capacity of a biosynthetic poly(vinyl alcohol) (PVA) hydrogel to support growth and differentiation of co-encapsulated neurons and glia. A significant challenge is to understand the role of the dynamic degradable hydrogel mechanical properties on expression of relevant cellular morphologies and function. It was hypothesised that a carrier with mechanical properties akin to neural tissue will provide glia with conditions to thrive, and that glia in turn will support neuronal survival and development. PVA copolymerised with biological macromolecules sericin and gelatin (PVA-SG) and with tailored nerve tissue-like mechanical properties were used to encapsulate Schwann cells (SCs) alone and subsequently a co-culture of SCs and neural-like PC12s. SCs were encapsulated within two PVA-SG gel variants with initial compressive moduli of 16 kPa and 2 kPa, spanning a range of reported mechanical properties for neural tissues. Both hydrogels were shown to support cell viability and expression of extracellular matrix proteins, however, SCs grown within the PVA-SG with a higher initial modulus were observed to present with greater physiologically relevant morphologies and increased expression of extracellular matrix proteins. The higher modulus PVA-SG was subsequently shown to support development of neuronal networks when SCs were co-encapsulated with PC12s. The lower modulus hydrogel was unable to support effective development of neural networks. This study demonstrates the critical link between hydrogel properties and glial cell phenotype on development of functional neural tissues.
机译：促进神经再生需要工程细胞载体以物理和生物化学地支持神经元生长至持久的功能组织。该研究系统地评估了生物合成的聚（乙烯醇）（PVA）水凝胶的能力，以支持共涂覆的神经元和胶质细胞的生长和分化。重大挑战是了解动态可降解水凝胶机械性能对相关细胞形态和功能表达的作用。假设具有类似于神经组织的机械性能的载体将提供巨大的细节，以茁壮成长，并且冰川将支持神经元生存和发育。用生物大分子硅蛋白和明胶（PVA-SG）共聚的PVA和具有定制的神经组织状机械性能，用于单独包封Schwann细胞（SCS），随后是SCS和神经状PC12s的共培养。 SCS在两个PVA-SG凝胶变体内包封，其初始压缩模量为16kPa和2kPa，跨越一系列报告的神经组织的机械性能。显示了两种水凝胶以支持细胞活力，并且细胞外基质蛋白的表达，然而，观察到具有较高初始模量的PVA-SG内生长的SC呈现出更大的生理学相关的形态和细胞外基质蛋白的表达增加。随后显示较高的模量PVA-SG以支持当SCS与PC12S共封装时的神经元网络的发展。下模量水凝胶无法支持神经网络的有效发展。该研究表明了水凝胶特性与胶质细胞表型对功能性神经组织发育的关键联系。

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