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首页> 外文期刊>Acta biomaterialia >Artificial ligament made from silk protein/Laponite hybrid fibers
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Artificial ligament made from silk protein/Laponite hybrid fibers

机译：由丝蛋白/丙酮杂交纤维制成的人造韧带

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With developments in tissue engineering, artificial ligaments are expected to be future materials for anterior cruciate ligament (ACL) reconstruction. However, poor healing of the intraosseous part after ACL reconstruction significantly hinders their applications in this field. In this study, a bioactive clay Laponite (LAP) was introduced into the regenerated silk fibroin (RSF) spinning dope to produce functional RSF/LAP hybrid fibers by wet-spinning. These RSF/LAP hybrid fibers were then woven into artificial ligament for ACL reconstruction. The structure and mechanical properties of RSF/LAP hybrid fibers were extensively studied by different means. Results confirmed the presence of LAP in RSF fibers and revealed that the addition of LAP slightly deteriorated the comprehensive mechanical properties of RSF fibers. However, they were still much tougher (with higher breaking energy) than those of degummed natural silkworm silk that was earlier used for making artificial ligament. The artificial ligament woven from RSF/LAP hybrid fibers showed better cytocompatibility and osteogenic differentiation with mouse pre-osteoblasts in vitro than those made from degummed natural silkworm silks and pure RSF fibers. Furthermore, in vivo study in a rat ACL reconstruction model demonstrated that the presence of LAP in the artificial ligament could significantly enhance the graft osseointegration process and also improve the corresponding biomechanical properties of the artificial ligament. Based upon these results, the RSF/LAP hybrid fibers, which can be mass produced by wet-spinning process, are believed to have a great potential for use as artificial ligament materials for ACL reconstruction.
机译：随着组织工程的发展，预计人造韧带将成为未来十字韧带（ACL）重建的未来材料。然而，在ACL重建后骨髓内部的愈合差异显着阻碍了它们在该领域的应用。在该研究中，将生物活性粘土丙酸盐（圈）引入再生丝素蛋白（RSF）纺丝涂料中，通过湿旋转产生功能性RSF / LAP杂交纤维。然后将这些RSF / LAP杂交纤维编织成用于ACL重建的人工韧带。通过不同的方法广泛地研究了RSF / LAP杂交纤维的结构和机械性能。结果证实了RSF纤维中的膝盖的存在，并揭示了膝盖的添加略微恶化了RSF纤维的综合机械性能。然而，它们仍然更加强硬（具有更高的断裂能量），而不是脱胶的天然蚕丝，早期用于制作人造韧带。从RSF / LAP杂交纤维编织的人造韧带均显示出比由脱胶天然蚕丝和纯RSF纤维制成的小鼠预成骨细胞的细胞膜相容性和骨质发生分化。此外，在大鼠ACL重建模型中的体内研究表明，人工韧带中的膝盖的存在可以显着增强接枝骨整合过程，并提高人造韧带的相应生物力学性质。基于这些结果，据认为，通过湿纺过程可以是诸如湿纺过程的质量的RSF / LAP杂化纤维具有巨大的用途作为ACL重建的人工韧带材料。

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