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首页> 外文期刊>Acta biomaterialia >Preparation of a collagen/polymer hybrid gel for tissue membranes. Part II: in vitro and in vivo biological properties of the collagen gels.
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Preparation of a collagen/polymer hybrid gel for tissue membranes. Part II: in vitro and in vivo biological properties of the collagen gels.

机译：组织膜的胶原/聚合物杂交凝胶的制备。 第二部分：体外和体内胶原凝胶的生物学性质。

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摘要

The aim of the present study was to evaluate the biological properties of a collagen-phospholipid polymer hybrid gel (MiC30 gel) designed for use as a tissue membrane. The following four types of collagen gels were synthesized and tested in vitro and in vivo: physically cross-linked collagen gel (Uc gel), N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC)/N-hydroxysuccinimide-cross-linked collagen gel (EN gel), MiC30 gel and glutaraldehyde-cross-linked collagen gel (G gel). The cell adhesivity and proliferation rate were observed to be lowest for the MiC30 gel and highest for the Uc gel, indicating that the phospholipid-polymer-covered surface of the collagen gel interacted weakly with cells. The collagen gel was implanted into rats subcutaneously and was observed 1, 2 and 8 weeks after implantation. The Uc gel and G gel were degraded and induced an inflammatory response. Granulation was not observed for 8 weeks after implantation and the formation of foreign body giant cells was observed around both the Uc and G gels. On the other hand, cell infiltration and degradation were not observed in the case of the EN and MiC30 gels. The formation of foreign body giant cells was suppressed and the healing process was accelerated. The MiC30 gel is suitable for use as a biomaterial that is stable in vivo because it suppresses the foreign body response and accelerates the healing process.
机译：本研究的目的是评估设计用于组织膜的胶原磷脂聚合物杂交凝胶（MIC30凝胶）的生物学性质。在体外和体内合成和测试以下四种类型的胶原凝胶：物理交联胶原凝胶（UC凝胶），N-（3-二甲基氨基丙基）-N'-乙基碳二亚胺（EDC）/ N-羟基琥珀酰亚胺 - 交叉 - 连接的胶原凝胶（EN凝胶），MIC30凝胶和戊二醛交联胶原凝胶（G凝胶）。观察到细胞粘附和增殖率为MIC30凝胶最低，并且对于UC凝胶最高，表明胶原凝胶的磷脂 - 聚合物覆盖表面与细胞弱相互作用。皮下将胶原凝胶皮下植入大鼠，并在植入后1,2和8周观察到。 UC凝胶和G凝胶降解并诱导炎症反应。在植入后8周未观察到颗粒，并且在UC和G凝胶周围观察到外部体巨细胞的形成。另一方面，在EN和MIC30凝胶的情况下未观察到细胞浸润和降解。抑制了异物巨细胞的形成，加速了愈合过程。 MIC30凝胶适合用作体内稳定的生物材料，因为它抑制了异物响应并加速愈合过程。

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  • 来源
    《Acta biomaterialia》 |2010年第2期|共9页
  • 作者

    Nam K; Kimura T; Funamoto;

  • 作者单位

    Division of Biofunctional Molecules Institute of Biomaterials and Bioengineering Tokyo Medical and Dental University Chiyoda-ku Tokyo Japan.;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物医学工程;
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