• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Visible light crosslinkable chitosan hydrogels for tissue engineering
【24h】

Visible light crosslinkable chitosan hydrogels for tissue engineering

机译：用于组织工程的可见光可交联壳聚糖水凝胶

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In situ gelling constructs, which form a hydrogel at the site of injection, offer the advantage of delivering cells and growth factors to the complex structure of the defect area for tissue engineering. In the present study, visible light crosslinkable hydrogel systems were presented using methacrylated glycol chitosan (MeGC) and three blue light initiators: camphorquinone (CQ), fluorescein (FR) and riboflavin (RF). A minimal irradiation time of 120 s was required to produce MeGC gels able to encapsulate cells with CQ or FR. Although prolonged irradiation up to 600 s improved the mechanical strength of CQ- or FR-initiated gels (compressive modulus 2.8 or 4.4 kPa, respectively), these conditions drastically reduced encapsulated chondrocyte viability to 5% and 25% for CQ and FR, respectively. Stable gels with 80-90% cell viability could be constructed using radiofrequency (RF) with only 40 s irradiation time. Increasing irradiation time up to 300 s significantly improved the compressive modulus of the RF-initiated MeGC gels up to 8.5 kPa without reducing cell viability. The swelling ratio and degradation rate were smaller at higher irradiation time. RF-photoinitiated hydrogels supported proliferation of encapsulated chondrocytes and extracellular matrix deposition. The feasibility of this photoinitiating system as in situ gelling hydrogels was further demonstrated in osteochondral and chondral defect models for potential cartilage tissue engineering. The MeGC hydrogels using RF offer a promising photoinitiating system in tissue engineering applications.
机译：在原位胶凝构建体中，在注射部位形成水凝胶，提供给组织工程缺陷区域的复杂结构的传递细胞和生长因素的优势。在本研究中，使用甲基丙烯酸甲基乙二醇壳聚糖（MEGC）和三个蓝光引发剂：樟脑醌（CQ），荧光素（FR）和核黄素（RF），给出可见光可交联水凝胶系统。产生120秒的最小照射时间来生产能够用CQ或Fr封装细胞的MEGC凝胶。虽然延长的辐射高达600秒改善了CQ-或FR引发的凝胶的机械强度（分别是压缩模量2.8或4.4kPa），但这些条件分别将包封的软骨细胞活力急剧下降至5％和25％的CQ和Fr。可以使用仅具有40秒的照射时间的射频（RF）来构建具有80-90％的细胞活力的稳定凝胶。增加辐照时间高达300秒显着改善了RF引发的MEGC凝胶的压缩模量高达8.5kPa而不降低细胞活力。在较高的照射时间下溶胀比和降解速率较小。 RF-Pheopinited水凝胶支持包封的软骨细胞和细胞外基质沉积的增殖。这种光引发系统的可行性在原位胶凝水凝胶中进一步证明了脑骨细胞和潜在软骨组织工程的缺陷模型。 Megc水凝胶使用RF提供了有希望的用于组织工程应用的光诱导系统。

著录项

  • 来源
    《Acta biomaterialia》 |2012年第5期|共9页
  • 作者

    HuJ.; HouY.; ParkH.; ChoiB.; HouS.; ChungA.; LeeM.;

  • 作者单位

    Division of Advanced Prosthodontics Biomaterials and Hospital Dentistry University of California;

    Division of Advanced Prosthodontics Biomaterials and Hospital Dentistry University of California;

    Division of Advanced Prosthodontics Biomaterials and Hospital Dentistry University of California;

    Division of Advanced Prosthodontics Biomaterials and Hospital Dentistry University of California;

    Biomedical Engineering Interdisciplinary Program University of California Los Angeles CA 90095;

    Biomedical Engineering Interdisciplinary Program University of California Los Angeles CA 90095;

    Division of Advanced Prosthodontics Biomaterials and Hospital Dentistry University of California;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 普通生物学;
  • 关键词

    Cell encapsulation; Chitosan; Hydrogel; Photopolymerization; Tissue engineering;

    机译：细胞包封;壳聚糖;水凝胶;光聚合;组织工程;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号