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首页> 外文期刊>Acta biomaterialia >Immediate production of a tubular dense collagen construct with bioinspired mechanical properties
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Immediate production of a tubular dense collagen construct with bioinspired mechanical properties

机译：用生物透露机械性能立即产生管状致密胶原构建体

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摘要

The intrinsic complexity of tissues and organs demands tissue engineering approaches that extend beyond planar constructs currently in clinical use. However, the engineering of cylindrical or tubular tissue constructs with a hollow lumen presents significant challenges arising from geometrical and architectural considerations required to tailor biomaterials for tissue and organ repair. Type I collagen is an ideal scaffolding material due to its outstanding biocompatibility and high processability. However, the highly hydrated nature of collagen hydrogels results in their lack of mechanical properties and instability, as well as extensive cell-mediated contraction, which must be overcome to achieve process control. Herein, tubular dense collagen constructs (TDCCs) were produced simply and rapidly (in less than 1 h) by circumferentially wrapping plastically compressed dense collagen gel sheets around a cylindrical support. The effects of collagen source, i.e. rat-tail tendon and bovine dermis-derived acid solubilized collagen, and concentration on TDCC properties were investigated through morphological, mechanical and chemical characterizations. Both tensile strength and apparent modulus correlated strongly with physiologically relevant collagen gel fibrillar densities. The clinical potential of TDCC as a tubular tissue substitute was demonstrated mechanically, through circumferential tensile properties, theoretical burst pressure, which ranged from 1225 to 1574 mm Hg, compliance values of between 8.3% to 14.2% per 100 mm Hg and suture retention strength in the range of 116-151 grams-force, which were compatible with surgical procedures. Moreover, NIH/3T3 fibroblast viability and uniform distribution within the construct wall were confirmed up to day 7 in culture. TDCCs with fibrillar densities equivalent to native tissues can be readily engineered in various dimensions with tunable morphological and mechanical properties, which can be easily handled for use as tissue models and adapted to clinical needs.
机译：组织和器官的内在复杂性要求组织工程方法延伸到目前在临床用途的平面构建体中。然而，具有中空腔的圆柱形或管状组织构建体的工程呈现出从用于组织和器官修复的生物材料所需的几何和架构考虑所产生的重大挑战。 I型胶原蛋白是一种理想的脚手架材料，由于其出色的生物相容性和高可加工性。然而，胶原水凝胶的高水合性质导致它们缺乏机械性能和不稳定性，以及广泛的细胞介导的收缩，这必须克服以实现过程控制。这里，通过围绕圆柱形支撑件周向包裹塑性压缩的致密胶原凝胶片，简单且快速地（小于1小时）的管状致密胶原构建体（TDCC）。通过形态学，机械和化学表征研究了胶原蛋白来源的影响，即RAT尾肌腱和牛衍生的溶液溶解胶原和TDCC性能的浓度。抗拉强度和表观模量均用生理相关的胶原凝胶纤维状密度强烈地相关。作为管状组织替代品的TDCC作为管状组织替代物的临床电位，通过周向拉伸性能，理论突发压力，范围为1225至1574mm HG，依从性值为每100 mm HG和缝合线滞留强度的8.3％至14.2％。 116-151克力的范围，与手术程序相容。此外，构建壁内的NIH / 3T3成纤维细胞活力和均匀分布在培养物中最多确认了第7天。具有相当于本地组织的纤维状密度的TDCC可以在各种尺寸中容易地设计成具有可调的形态和机械性能，这可以很容易地处理用作组织模型并适应临床需求。

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  • 来源
    《Acta biomaterialia》 |2012年第5期|共13页
  • 作者

    GhezziC.E.; MarelliB.; MujaN.; NazhatS.N.;

  • 作者单位

    Department of Mining and Materials Engineering McGill University 3610 University Street Montréal;

    Department of Mining and Materials Engineering McGill University 3610 University Street Montréal;

    Department of Mining and Materials Engineering McGill University 3610 University Street Montréal;

    Department of Mining and Materials Engineering McGill University 3610 University Street Montréal;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 普通生物学;
  • 关键词

    Compliance; Plastic compression; Tissue model; Tube; Vascular;

    机译：遵守;塑料压缩;组织模型;管;血管;

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