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首页> 外文期刊>Acta biomaterialia >Synergistic effect of surface modification and scaffold design of bioplotted 3-D poly-ε-caprolactone scaffolds in osteogenic tissue engineering.
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Synergistic effect of surface modification and scaffold design of bioplotted 3-D poly-ε-caprolactone scaffolds in osteogenic tissue engineering.

机译：成骨组织工程中生物培育3-D多ε-己内酯支架的表面改性和支架设计的协同作用。

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摘要

The hydrophobic nature and the regular scaffold architecture of bioplotted poly(ε-caprolactone) (PCL) scaffolds present some hurdles for homogeneous tissue formation and differentiation. The current hypothesis is that a synergistic effect of applied surface modification and scaffold design enhances colonization and osteogenic differentiation. First, PCL scaffolds with a 0/90° lay-down pattern (0/90) were plotted and subjected to an oxygen plasma (O2) or multistep surface modification, including post-argon 2-amino-ethylmethacrylate grafting (AEMA), followed by immobilization of gelatin type B (gelB) and physisorption of fibronectin (gelB Fn). Secondly, scaffolds of different designs were plotted (0/90° shift (0/90 S), 0/45° and 0/90° with narrow pores (0/90 NP)) and subjected to the double protein coating. Preosteoblasts were cultured on the scaffolds and the seeding efficiency, colonization and differentiation were studied. The data revealed that a biomimetic surface modification improved colonization (gelB Fn>gelB>AEMA>O2). Compact scaffold architectures (0/90 NP, 0/45, 0/90 S>0/90) positively influenced the seeding efficiency and differentiation. Interestingly, the applied surface modification had a greater impact on colonization than the scaffold design. In conclusion, the combination of a double protein coating with a compact design enhances tissue formation in the plotted PCL scaffolds.
机译：疏水性质和生物培养的聚（ε-己内酯）（PCL）支架的常规支架结构存在一些障碍用于均匀组织形成和分化。目前的假设是应用表面改性和支架设计的协同效应增强了定植和骨质发生分化。首先，绘制具有0/90°左下图案（0/90）的PCL支架并进行氧等离子体（O2）或多体表面改性，包括后氩的2-氨基 - 乙基丙烯酸酯接枝（AEMA），然后进行通过固定明胶型B（GELB）和纤连蛋白的理由（GELB FN）。其次，绘制了不同设计的支架（0/90°Shift（0/90s），0/45°和0/90°，狭窄的孔（0/90 NP））并进行双蛋白质涂层。在支架上培养了预细胞细胞，研究了种子效率，定植和分化。数据显示，仿生表面改性改善了定植（GELB FN> GELB> AEMA> O2）。紧凑的脚手架架构（0/90 NP，0 / 45,0 / 90 s> 0/90）积极影响播种效率和分化。有趣的是，应用的表面改性对殖民化的影响大于支架设计。总之，双蛋白质涂层的组合具有紧凑的设计，增强了绘制的PCL支架中的组织形成。

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