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首页> 外文期刊>Acta biomaterialia >Improving protein delivery of fibroblast growth factor-2 from bacterial inclusion bodies used as cell culture substrates
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Improving protein delivery of fibroblast growth factor-2 from bacterial inclusion bodies used as cell culture substrates

机译：从用作细胞培养物基材的细菌包涵体改善成纤维细胞生长因子-2的蛋白质递送

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摘要

Bacterial inclusion bodies (IBs) have recently been used to generate biocompatible cell culture interfaces, with diverse effects on cultured cells such as cell adhesion enhancement, stimulation of cell growth or induction of mesenchymal stem cell differentiation. Additionally, novel applications of IBs as sustained protein delivery systems with potential applications in regenerative medicine have been successfully explored. In this scenario, with IBs gaining significance in the biomedical field, the fine tuning of this functional biomaterial is crucial. In this work, the effect of temperature on fibroblast growth factor-2 (FGF-2) IB production and performance has been evaluated. FGF-2 was overexpressed in Escherichia coli at 25 and 37 C, producing IBs with differences in size, particle structure and biological activity. Cell culture topographies made with FGF-2 IBs biofabricated at 25 C showed higher levels of biological activity as well as a looser supramolecular structure, enabling a higher protein release from the particles. In addition, the controlled use of FGF-2 protein particles enabled the generation of functional topographies with multiple biological activities being effective on diverse cell types.
机译：最近用于产生生物相容性细胞培养界面的细菌包涵体（IBS），对培养细胞如细胞粘附增强，刺激细胞生长或间充质干细胞分化的诱导作用不同。另外，已经成功地探讨了IBS作为持续蛋白质递送系统的新型应用，具有潜在的再生医学应用。在这种情况下，在生物医学领域获得了IBS，这种功能生物材料的微调至关重要。在这项工作中，已经评估了温度对成纤维细胞生长因子-2（FGF-2）IB产生和性能的影响。 FGF-2在25和37℃的大肠杆菌中过表达，产生具有差异大小，颗粒结构和生物活性的IBS。用FGF-2 IBS生物制衡的细胞培养地形在25℃下表现出更高水平的生物活性以及松散的超分子结构，使得从颗粒中释放出更高的蛋白质。此外，对FGF-2蛋白质颗粒的受控使用使得具有多种生物学活性的功能性地形，这些生物学活性有效地对不同的细胞类型有效。

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  • 来源
    《Acta biomaterialia》 |2014年第3期|共6页
  • 作者

    Seras-FranzosoJ.; PeeboK.; García-FruitósE.; VázquezE.; RinasU.; VillaverdeA.;

  • 作者单位

    Institut de Biotecnologia i de Biomedicina Universitat Autònoma de Barcelona Bellaterra 08193;

    Competence Centre of Food and Fermentation Technologies Akadeemia tee 15a 12618 Tallinn Estonia;

    Institut de Biotecnologia i de Biomedicina Universitat Autònoma de Barcelona Bellaterra 08193;

    Institut de Biotecnologia i de Biomedicina Universitat Autònoma de Barcelona Bellaterra 08193;

    Institute of Technical Chemistry-Life Science Leibniz University of Hannover D-30167 Hannover;

    Institut de Biotecnologia i de Biomedicina Universitat Autònoma de Barcelona Bellaterra 08193;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 普通生物学;
  • 关键词

    Drug delivery; Inclusion bodies; Nanomedicine; Protein release; Surface functionalization;

    机译：药物递送;包涵体;纳米医生;蛋白质释放;表面官能化;

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