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首页> 外文期刊>Acta biomaterialia >Mechanostructure and composition of highly reproducible decellularized liver matrices.
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Mechanostructure and composition of highly reproducible decellularized liver matrices.

机译：机械结构和高度可重复脱细胞肝矩阵的组成。

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Despite the increasing number of papers on decellularized scaffolds, there is little consensus on the optimum method of decellularizing biological tissue such that the micro-architecture and protein content of the matrix are conserved as far as possible. Focusing on the liver, the aim of this study was therefore to develop a method for the production of well-characterized and reproducible matrices that best preserves the structure and composition of the native extra cellular matrix (ECM). Given the importance of matrix stiffness in regulating cell response, the mechanical properties of the decellularized tissue were also considered. The testing and analysis framework is based on the characterization of decellularized and untreated samples in the same reproducible initial state (i.e., the equilibrium swollen state). Decellularized ECM (dECM) were characterized using biochemical, histological, mechanical and structural analyses to identify the best procedure to ensure complete cell removal while preserving most of the native ECM structure and composition. Using this method, sterile decellularized porcine ECM with highly conserved intra-lobular micro-structure and protein content were obtained in a consistent and reproducible manner using the equilibrium swollen state of tissue or matrix as a reference. A significant reduction in the compressive elastic modulus was observed for liver dECM with respect to native tissue, suggesting a re-examination of design parameters for ECM-mimicking scaffolds for engineering tissues in vitro.
机译：尽管越来越多的渐变支架上的纸张，但对脱细胞化生物组织的最佳方法几乎没有共识，使得基质的微观结构和蛋白质含量尽可能地保守。因此，专注于肝脏，本研究的目的是开发一种生产良好的具有特征和可重复的基质的方法，该矩阵最能保留天然额外细胞基质（ECM）的结构和组成。鉴于基质刚度在调节细胞响应方面的重要性，还考虑了脱细胞化组织的机械性能。测试和分析框架基于同一可再现初始状态（即平衡肿胀状态）的脱细胞化和未处理样品的表征。使用生化，组织学，机械和结构分析表征脱细胞的ECM（DECM），以确定确保完全细胞的最佳方法，同时保留大多数本地ECM结构和组合物。使用该方法，使用均衡的组织或基质的平衡状态或基质作为参考，以一致和可重复的方式获得具有高度保守的小叶微结构和蛋白质含量的无菌脱细胞猪ECM。对于肝脏DECM，对天然组织的肝脏DECM观察到压缩弹性模量的显着降低，表明在体外用于工程组织的ECM模拟支架的设计参数重新检查。

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