• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering
【24h】

Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering

机译：Langmuir-Schaefer薄膜沉积到蜂窝多孔膜上，用于视网膜组织工程

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Age-related macular degeneration (AMD) is the leading cause of vision loss in senior citizens in the developed world. The disease is characterised by the degeneration of a specific cell layer at the back of the eye - the retinal pigment epithelium (RPE), which is essential in retinal function. The most promising therapeutic option to restore the lost vision is considered to be RPE cell transplantation. This work focuses on the development of biodegradable biomaterials with similar properties to the native Bruch's membrane as carriers for RPE cells. In particular, the breath figure (BF) method was used to create semi-permeable microporous films, which were thereafter used as the substrate for the consecutive Langmuir-Schaefer (LS) deposition of highly organised layers of collagen type I and collagen type IV. The newly developed biomaterials were further characterised in terms of surface porosity, roughness, hydrophilicity, collagen distribution, diffusion properties and hydrolytic stability. Human embryonic stem cell-derived RPE cells (hESC-RPE) cultured on the biomaterials showed good adhesion, spreading and morphology, as well as the expression of specific protein markers. Cell function was additionally confirmed by the assessment of the phagocytic capacity of hESC-RPE. Throughout the study, microporous films consistently showed better results as cell culture materials for hESC-RPE than dip-coated controls. This work demonstrates the potential of the BF-LS combined technologies to create biomimetic prosthetic Bruch's membranes for hESC-RPE transplantation.
机译：与年龄相关的黄斑变性（AMD）是发达国家老年人视力损失的主要原因。该疾病的特征在于眼睛背面的特定细胞层的退化 - 视网膜颜料上皮（RPE），这对于视网膜功能至关重要。恢复丧失视力最有前途的治疗选择被认为是RPE细胞移植。这项工作侧重于在天然Bruch的膜中的可生物降解生物材料的发展，作为RPE细胞的载体。特别地，呼吸图（BF）方法用于产生半透透过的微孔膜，其后用作连续Langmuir-Schaefer（LS）沉积的胶原型I和胶原型IV型的基板。新开发的生物材料在表面孔隙率，粗糙度，亲水性，胶原分布，扩散性和水解稳定性方面进一步表征。在生物材料上培养的人胚胎干细胞衍生的RPE细胞（HESC-RPE）显示出良好的粘附性，扩散和形态，以及特定蛋白质标志物的表达。另外通过评估HESC-RPE的吞噬能力来证实细胞功能。在整个研究中，微孔薄膜始终显示出比浸涂对照组的HESC-RPE的细胞培养材料更好的结果。这项工作展示了BF-LS组合技术的潜力，以创建用于HESC-RPE移植的仿生假体Bruch的膜。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号