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首页> 外文期刊>Acta biomaterialia >Selective effect of hydroxyapatite nanoparticles on osteoporotic and healthy bone formation correlates with intracellular calcium homeostasis regulation
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Selective effect of hydroxyapatite nanoparticles on osteoporotic and healthy bone formation correlates with intracellular calcium homeostasis regulation

机译：羟基磷灰石纳米粒子对骨质疏松和健康骨形成的选择性效果与细胞内钙稳态调节相关

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摘要

Adequate bone substitutes osseointegration has been difficult to achieve in osteoporosis. Hydroxyapatite of the osteoporotic bone, secreted by pathologic osteoblasts, had a smaller crystal size and lower crystallinity than that of the normal. To date, little is known regarding the interaction of synthetic hydroxyapatite nanoparticles (HANPs) with osteoblasts born in bone rarefaction. The present study investigated the biological effects of HANPs on osteoblastic cells derived from osteoporotic rat bone (OVX-OB), in comparison with the healthy ones (SHM-OB). A selective effect of different concentrations of HANPs on the two cell lines was observed that the osteoporotic osteoblasts had a higher tolerance. Reductions in cell proliferation, ALP activity, collagen secretion and osteoblastic gene expressions were found in the SHM-OB when administered with HANPs concentration higher than 25 mu g/ml. In contrast, those of the OVX-OB suffered no depression but benefited from 25 to 250 mu g/ml HANPs in a dose-dependent manner. We demonstrated that the different effects of HANPs on osteoblasts were associated with the intracellular calcium influx into the endoplasmic reticulum. The in vivo bone defect model further confirmed that, with a critical HANPs concentration administration, the osteoporotic rats had more and mechanically matured new bone formation than the non-treated ones, whilst the sham rats healed no better than the natural healing control. Collectively, the observed epigenetic regulation of osteoblastic cell function by HANPs has significant implication on defining design parameters for a potential therapeutic use of nanomaterials.
机译：足够的骨代替骨整合难以在骨质疏松症中实现。通过病理成骨细胞分泌的骨质疏松骨的羟基磷灰石具有较小的晶体尺寸和较低的结晶性而不是正常的。迄今为止，关于合成羟基磷灰石纳米颗粒（HANP）与骨质细胞的相互作用很少，以骨稀疏的骨质细胞相互作用。本研究研究了汉普人对骨科大鼠骨（OVX-OB）衍生自骨质细胞的生物学作用，与健康（SHM-OB）相比。观察到不同浓度HANP对两种细胞系的选择性效果，骨质疏松骨质细胞具有更高的耐受性。在SHM-OB中施用高于25μg/ mL的HANPS浓度时，在SHM-OB中发现细胞增殖，ALP活性，胶原分泌和骨细胞基因表达的降低。相反，ovx-ob的那些患有抑郁症，但以剂量依赖性方式从25至250μg/ ml Hanps中受益。我们证明，HANP对成骨细胞的不同效果与细胞内钙流入内质网的细胞细胞相关。在体内骨缺陷模型进一步证实，通过临界汉普人浓度给药，骨质疏松大鼠的新骨形成多于未治疗的骨形成，而假大鼠愈合而不是自然愈合控制更好。总的来说，HANPS的观察到的骨细胞功能函数的表观遗传调节对纳米材料潜在治疗使用的设计参数有显着意义。

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  • 来源
    《Acta biomaterialia》 |2017年第2017期|共13页
  • 作者

    Zhao Rui; Xie Pengfei; Zhang Kun; Tang Zhurong; Chen Xuening; Zhu Xiangdong; Fan Yujiang; Yang Xiao; Zhang Xingdong;

  • 作者单位

    Sichuan Univ Natl Engn Res Ctr Biomat 29 Wangjiang Rd Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Natl Engn Res Ctr Biomat 29 Wangjiang Rd Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Natl Engn Res Ctr Biomat 29 Wangjiang Rd Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Natl Engn Res Ctr Biomat 29 Wangjiang Rd Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Natl Engn Res Ctr Biomat 29 Wangjiang Rd Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Natl Engn Res Ctr Biomat 29 Wangjiang Rd Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Natl Engn Res Ctr Biomat 29 Wangjiang Rd Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Natl Engn Res Ctr Biomat 29 Wangjiang Rd Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Natl Engn Res Ctr Biomat 29 Wangjiang Rd Chengdu 610064 Sichuan Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 普通生物学;
  • 关键词

    Hydroxyapatite nanoparticles; Osteoporotic osteoblast; Concentration; Osseointegration; Intracellular calcium;

    机译：羟基磷灰石纳米颗粒;骨质疏松骨质细胞;浓度;骨整合;细胞内钙;

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