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首页> 外文期刊>Acta biomaterialia >Cross-linking of a biopolymer-peptide co-assembling system
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Cross-linking of a biopolymer-peptide co-assembling system

机译：生物聚合物肽共组装系统的交联

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The ability to guide molecular self-assembly at the nanoscale into complex macroscopic structures could enable the development of functional synthetic materials that exhibit properties of natural tissues such as hierarchy, adaptability, and self-healing. However, the stability and structural integrity of these kinds of materials remains a challenge for many practical applications. We have recently developed a dynamic biopolymer-peptide co-assembly system with the capacity to grow and undergo morphogenesis into complex shapes. Here we explored the potential of different synthetic (succinimidyl carboxymethyl ester, poly (ethylene glycol) ether tetrasuccinimidyl glutarate and glutaraldehyde) and natural (genipin) cross linking agents to stabilize membranes made from these biopolymer-peptide co-assemblies. We investigated the cross-linking efficiency, resistance to enzymatic degradation, and mechanical properties of the different cross-linked membranes. We also compared their biocompatibility by assessing the metabolic activity and morphology of adipose-derived stem cells (ADSC) cultured on the different membranes. While all cross-linkers successfully stabilized the system under physiological conditions, membranes cross-linked with genipin exhibited better resistance in physiological environments, improved stability under enzymatic degradation, and a higher degree of in vitro cytocompatibility compared to the other cross-linking agents. The results demonstrated that genipin is an attractive candidate to provide functional structural stability to complex self-assembling structures for potential tissue engineering or in vitro model applications.
机译：将分子自组装在纳米级中指导复合宏观结构的能力可以使功能性合成材料的发育表现出自然组织的性质，例如等级，适应性和自我愈合。然而，这些材料的稳定性和结构完整性对于许多实际应用仍然是一个挑战。我们最近开发了一种动态的生物聚合物 - 肽共组装系统，其能力生长并经过形态发生成复杂的形状。在这里，我们探讨了不同合成（琥珀酰亚胺羧甲基酯，聚（乙二醇）醚四琥珀酰亚胺氨基甲基氨基甲酰胺和戊二醛）和天然（Genipin）交联试剂的潜力，以稳定由这些生物聚合物肽共组件制成的膜。我们研究了交联效率，抗酶降解，以及不同交联膜的机械性能。我们还通过评估在不同膜上培养的脂肪衍生的干细胞（ADSC）的代谢活性和形态来比较其生物相容性。虽然所有交联剂在生理条件下成功地稳定了系统，但与Genipin交联的膜在生理环境中表现出更好的抵抗力，并在酶促降解下提高稳定性，与其他交联剂相比具有更高程度的体外细胞相容性。结果表明，Genipin是一种有吸引力的候选者，为潜在的组织工程或体外模型应用提供复杂的自组装结构的功能结构稳定性。

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