• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Microsphere-based scaffolds for cartilage tissue engineering: using subcritical CO(2) as a sintering agent.
【24h】

Microsphere-based scaffolds for cartilage tissue engineering: using subcritical CO(2) as a sintering agent.

机译：用于软骨组织工程的微球基支架：使用亚临界CO（2）作为烧结剂。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Shape-specific, macroporous tissue engineering scaffolds were fabricated and homogeneously seeded with cells in a single step. This method brings together CO(2) polymer processing and microparticle-based scaffolds in a manner that allows each to solve the key limitation of the other. Specifically, microparticle-based scaffolds have suffered from the limitation that conventional microsphere sintering methods (e.g., heat, solvents) are not cytocompatible, yet we have shown that cell viability was sustained with subcritical (i.e., gaseous) CO(2) sintering of microspheres in the presence of cells at near-ambient temperatures. On the other hand, the fused microspheres provided the pore interconnectivity that has eluded supercritical CO(2) foaming approaches. Here, fused poly(lactide-co-glycolide) microsphere scaffolds were seeded with human umbilical cord mesenchymal stromal cells to demonstrate the feasibility of utilizing these matrices for cartilage regeneration. We also demonstrated that the approach may be modified to produce thin cell-loaded patches as a promising alternative for skin tissue engineering applications.
机译：特异性的大孔组织工程支架并在单一步骤中用细胞制造和均匀地播种。该方法以允许各自解决对方的关键限制的方式赋予CO（2）聚合物加工和基于微粒的支架。具体地，基于微粒的支架已经遭受限制，即常规的微球烧结方法（例如，热量，溶剂）不是细胞锁定的，但我们已经表明，细胞活力用亚临界（即，气态）CO（2）微球烧结来维持在近乎环境温度的细胞存在下。另一方面，熔融的微球提供了具有拼接超临界CO（2）发泡方法的孔互连性。这里，融合聚（丙交酯 - 共乙酰胺）微球支架与人脐部间充质基质细胞接种，以证明利用这些基质进行软骨再生的可行性。我们还证明，可以修饰该方法以产生薄的细胞加载的贴剂作为皮肤组织工程应用的有希望的替代方案。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号