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首页> 外文期刊>Acta biomaterialia >Spatial organization of osteoblast fibronectin matrix on titanium surfaces: effects of roughness, chemical heterogeneity and surface energy.
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Spatial organization of osteoblast fibronectin matrix on titanium surfaces: effects of roughness, chemical heterogeneity and surface energy.

机译：钛表面骨质细胞纤连蛋白基质的空间组织：粗糙度，化学异质性和表面能的影响。

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摘要

We investigated the early events of bone matrix formation, and specifically the role of fibronectin (FN) in the initial osteoblast interaction and the subsequent organization of a provisional FN matrix on different rough titanium (Ti) surfaces. Fluorescein isothiocyanate-labelled FN was preadsorbed on these surfaces and studied for its three-dimensional (3-D) organization by confocal microscopy, while its amount was quantified after NaOH extraction. An irregular pattern of adsorption with a higher amount of protein on topographic peaks than on valleys was observed and attributed to the physicochemical heterogeneity of the rough Ti surfaces. MG63 osteoblast-like cells were further cultured on FN-preadsorbed Ti surfaces and an improved initial cellular interaction was observed with increasing roughness. 3-D reconstruction of the immunofluorescence images after 4 days of incubation revealed that osteoblasts deposit FN fibrils in a specific facet-like pattern that is organized within the secreted total matrix overlying the top of the samples. The thickness of this FN layer increased when the roughness of the underlying topography was increased, but not by more than half of the total maximum peak-to-valley distance, as demonstrated with images showing simultaneous reconstruction of fluorescence and topography after 7 days of cell culture.
机译：我们研究了骨基质形成的早期事件，具体是纤维连接蛋白（Fn）在初始成骨细胞相互作用中的作用和随后的不同粗糙钛（Ti）表面上的临时Fn基质的作用。荧光素异硫氰酸酯标记的Fn在这些表面上预先吸收，并通过共聚焦显微镜研究其三维（3-D）组织，而NaOH提取后量化量。观察到具有较高量的蛋白质在谷物上的蛋白质的不规则吸附模式，并归因于粗糙Ti表面的物理化学异质性。在Fn-Pread吸收的Ti表面上进一步培养Mg63骨细胞样细胞，并且随着粗糙度的增加，观察到改善的初始细胞相互作用。孵育4天后的免疫荧光图像的3-D重建显示，成骨细胞以特定的小刻面样图案沉积Fn原纤维，其在分泌的总基质内组织在样品的顶部。当潜在地形的粗糙度增加而不是总最大峰到谷距离的粗糙度而增加，该Fn层的厚度增加，如图7天细胞7天后显示出同时重建荧光和地形的图像文化。

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