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首页> 外文期刊>Acta biomaterialia >Hypoxia influences the effects of magnesium degradation products on the interactions between endothelial and mesenchymal stem cells
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Hypoxia influences the effects of magnesium degradation products on the interactions between endothelial and mesenchymal stem cells

机译：缺氧影响镁降解产物对内皮和间充质干细胞之间相互作用的影响

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Biodegradable materials like well-documented Magnesium (Mg) are promising for their biocompatibility and tissue regeneration. Since Mg degradation is reported to be oxygen related, the effects of Mg were hypothesised to be influenced by oxygen. As two vital components of bone marrow, endothelial cells (EC) and mesenchymal stem cells (MSC), their interactions represent high scientific interest for tissue engineering and biodegradable Mg application. Human umbilical cord perivascular (HUCPV) and umbilical vein endothelial cell (HUVEC) were selected as sources of MSC and EC, respectively. Two types of coculture models were established to represent different phases of MSC-EC interaction: (i) where cells were physically separated thanks to a transwell and (ii) where cells were allowed to have heterotypic cellular contacts. Cell migration, gene, cytokines, and proliferation were investigated in HUCPV-HUVEC coculture using DNA, flow cytometry, wound healing assay, semi-quantitative real-time polymerase chain reaction (qRT-PCR), and enzyme-linked immunosorbent assay (ELISA). Mg degradation products increased HUCPV migration in transwell under hypoxia. Oxygen tension changed the gene regulation of migratory, angiogenetic or osteogenic regulators. Under contacting coculture and hypoxia, Mg degradation products remarkably increased cytokines (e.g., c-c motif chemokine ligand 2 and vascular endothelial growth factor) and MSC mineralisation. Mg degradation products decreased and increased the MSC proliferation in transwell and in heterotypic-contact coculture, respectively. In summary, this study indicates the roles of low oxygen and heterotypic contact to effects of Mg materials facilitating HUVEC and HUCPV.
机译：像记录良好的镁（Mg）这样的可生物降解的材料是对其生物相容性和组织再生的希望。由于据报道Mg降解是氧有关的，假设Mg的影响受氧气的影响。作为骨髓，内皮细胞（EC）和间充质干细胞（MSC）的两个重要组分，它们的相互作用代表了组织工程和可生物降解的MG应用的高科学兴趣。选择人的脐带羽毛状（HUCPV）和脐静脉内皮细胞（HUVEC）分别为MSC和EC的来源。建立了两种类型的共核模型以代表MSC-EC相互作用的不同阶段：（i）由于Transwlow和（II），其中允许细胞具有异型细胞接触的细胞物理分离。使用DNA，流式细胞术，伤口愈合测定，半定量实时聚合酶链反应（QRT-PCR）和酶联免疫吸附测定（ELISA）中研究了细胞迁移，基因，细胞因子和增殖。 。 MG降解产品在缺氧下的Transwell中增加了Hucpv迁移。氧气张力改变了迁移，血管生成或成骨调节剂的基因调节。在接触共培养和缺氧，Mg降解产物显着增加细胞因子（例如，C-C主题趋化因子配体2和血管内皮生长因子）和MSC矿化。 Mg降解产物分别降低并增加了Transwell和异质接触共培养的MSC增殖。总之，本研究表明低氧和异型接触的作用与促进HUVEC和HUCPV的Mg材料的影响。

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