首页> 外文期刊>Acta Crystallographica, Section B. Structural science, crystal engineering and materials >Inelastic scattering and solvent scattering reduce dynamical diffraction in biological crystals
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Inelastic scattering and solvent scattering reduce dynamical diffraction in biological crystals

机译:无弹性散射和溶剂散射减少生物晶体中的动力衍射

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摘要

Multi-slice simulations of electron diffraction by three-dimensional protein crystals have indicated that structure solution would be severely impeded by dynamical diffraction, especially when crystals are more than a few unit cells thick. In practice, however, dynamical diffraction turned out to be less of a problem than anticipated on the basis of these simulations. Here it is shown that two scattering phenomena, which are usually omitted from multi-slice simulations, reduce the dynamical effect: solvent scattering reduces the phase differences within the exit beam and inelastic scattering followed by elastic scattering results in diffusion of dynamical scattering out of Bragg peaks. Thus, these independent phenomena provide potential reasons for the apparent discrepancy between theory and practice in protein electron crystallography.
机译:通过三维蛋白质晶体的电子衍射的多切片模拟表明,通过动态衍射将严重阻抗结构溶液,特别是当晶体大于几个单位细胞厚时。 然而,在实践中,动态衍射结果不如这些模拟的基础预期的问题。 这里表明,两种散射现象通常从多切片模拟中省略,降低了动态效果:溶剂散射降低了出口梁和无弹性散射的相差,然后弹性散射导致动力散射出来的布拉格的扩散 山峰。 因此,这些独立的现象提供了蛋白质电子晶体学中理论与实践之间表观差异的潜在原因。

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