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首页> 外文期刊>Cytokine >Therapeutic effects of vaccine derived from amastigote surface protein-2 (ASP-2) against Chagas disease in mouse liver
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Therapeutic effects of vaccine derived from amastigote surface protein-2 (ASP-2) against Chagas disease in mouse liver

机译：疫苗衍生自Amastigotope蛋白-2（ASP-2）的疫苗对小鼠肝脏的Chagas病

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This study investigated the efficacy of the vaccine in liver of mice infected with the Trypanosoma cruzi (T. cruzi) and immunized with AdASP-2. For this purpose, histopathological analysis and gene expression of COX-2, TNFalpha, TNFR, iNOS, cytochrome C, caspase-3, TLR4, IL-6 and IL10 were evaluated. The following groups were used in this study: Group 1- Control Group (CTRL) animals received Adj3Gal vehicle; Group 2- Infected Group (TC) animals were infected with T. cruzi; Group 3- Immunized Group (AdASP-2): animals were immunized by AdASP-2 vaccine; Group 4- Immunized and Infected Group (AdASP-2 + TC) animals were infected with T. cruzi and immunized by AdSP-2 vaccine. A significant decrease of amastigote nests was noticed in the group of animals that were immunized with AdASP-2 and infected on the same day. COX-2 and TNF-alpha gene expressions increased in TC group, whereas TNF-alpha decreased in the TC + AdASP-2 group. TNFR expression was high in AdASP-2 + TC group. iNOS expression was high for all experimental groups whereas cytochrome C decreased for all experimental groups. Caspase 3 increased in TC and TC + AdASP-2 groups. The gene expression of TLR4 and IL-10 showed an increase in AdASP-2 + TC group. Finally, hepatic fibrosis was noticed to TC and AdASP-2 + TC groups. Taken together, our results demonstrated that vaccination with AdASP-2 was effective against the acute phase of experimental Chagas disease as a result of a more powerful and rapid immune response closely related to expression of some inflammatory genes, such as iNOS, TNF-alpha, TLR 4, and IL-10.
机译：本研究调查了疫苗在患有锥虫瘤Cruzi（T.Cruzi）的小鼠肝脏中的疗效，并用Adasp-2免疫。为此目的，评估COX-2，TNFalpha，TNFR，InOS，细胞色素C，Caspase-3，TLR4，IL-6和IL10的组织病理学分析和基因表达。本研究使用以下基团：第1组 - 对照组（CTRL）动物接受了add3gal车辆;第2组感染的群体（TC）动物被T.Cruzi感染;第3组免疫组（Adasp-2）：通过Adasp-2疫苗免疫动物;第4组免疫和感染的组（AdaSp-2 + Tc）动物被T.Cruzi感染并通过Adsp-2疫苗免疫。在用AdaSp-2免疫的动物组中注意到Amastigotoots Nests的显着降低，并在同一天感染。 TC组中COX-2和TNF-α基因表达增加，而TNF-α在TC + ADASP-2组中降低。 TNFR表达在AdaSP-2 + TC组中高。所有实验组的InOS表达高，而所有实验组的细胞色素C降低。 Caspase 3在TC和TC + AdaSP-2组中增加。 TLR4和IL-10的基因表达显示ADASP-2 + TC组增加。最后，注意到TC和Adasp-2 + TC组对肝纤维化。我们的结果表明，由于与一些炎症基因的表达密切相关的更强大和快速的免疫应答，与AdaSp-2的疫苗接种是有效的，这是对实验性噬菌体疾病的急性阶段的急性期。 TLR 4和IL-10。

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