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首页> 外文期刊>Cytokine >Association of interleukin (IL)-4 variable number of tandem repeats (VNTRs) and IL-4-590 promoter polymorphisms with susceptibility to pediatric autoimmune hepatitis type 1
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Association of interleukin (IL)-4 variable number of tandem repeats (VNTRs) and IL-4-590 promoter polymorphisms with susceptibility to pediatric autoimmune hepatitis type 1

机译:白细胞介素(IL)-4可变数量的串联重复(VNTRS)和IL-4-590启动子多态性与儿科自身免疫性肝炎1型易感性的促进剂多态性

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ObjectiveAutoimmune hepatitis (AIH) is a chronic inflammatory liver disease mediated by an autoimmune reaction to hepatocytes, the present study aimed to assess the possible associations between interleukin-4 (IL-4) variable number of tandem repeats (VNTRs) and IL-4-590 promoter polymorphisms and susceptibility to autoimmune hepatitis type 1 in children. Subjects and methodsThe study was performed on 101 children diagnosed with AIH and 104 apparently healthy, age and sex-matched control children, diagnosis of AIH was based on the simplified score for the diagnosis of AIH. Genotyping for the IL-4 VNTR and IL-4-590 were performed using PCR-RFLP. ResultsThe distribution of genotype frequencies of IL-4 gene intron 3 VNTR polymorphism were not significantly different between AIH patients and controls for 3R/2R and 2R/2R genotypes, while the 2R allele distribution was significantly higher among AIH patients than the control group. The frequency of IL-4-590 single nucleotide polymorphism (SNP) CT and TT genotypes was statistically higher among AIH patients than controls. ConclusionThis study revealed the presence of an association between IL-4 -590 TT genotype and T alleles with increased AIH risk in pediatric patients, also assess its severity as they were detected with Child Plugh scores B and C.
机译:客观的自身免疫肝炎(AIH)是由对肝细胞的自身免疫反应介导的慢性炎症性肝病,本研究旨在评估白细胞介素-4(IL-4)可变数量(VNTRS)和IL-4之间的可能关联。 590促进剂多态性和对儿童自身免疫性肝炎1型的易感性。关于诊断为AIH的101名儿童进行主题和方法研究表明,104例显然健康,年龄和性匹配的对照儿童,AIH的诊断基于AIH诊断的简化分数。使用PCR-RFLP进行IL-4 VNTR和IL-4-590的基因分型。 IL-4基因Intron 3 VNTR多态性基因型频率的分布在AIH患者和3r / 2R / 2R / 2R基因型的对照之间没有显着差异,而AIH患者的2R等位基因分布显着高于对照组。 IL-4-590单核苷酸多态性(SNP)CT和TT基因型的频率在AIH患者中统计学上高于对照。结论研究表明,在儿科患者的风险增加,IL-4 -590 TT基因型和T等位基因之间存在关联的存在,也评估其严重程度,因为它们被儿童PLUGH评分B和C.

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