...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cytokine >Transplantation of mesenchymal stem cells into the skeletal muscle induces cytokine generation
【24h】

Transplantation of mesenchymal stem cells into the skeletal muscle induces cytokine generation

机译：间充质干细胞移植到骨骼肌中诱导细胞因子一代

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mesenchymal stem cells due to the high proliferative potential, capacity of multilineage differentiation became a hope of regenerative medicine. However, the organism's response to the transplantation of MSCs is not fully elucidated. The aim of the present study was to evaluate the acute local tissue response to syngeneic MSCs administration into the muscle. Rat syngeneic MSCs were transplanted into the skeletal muscle and the tissue surrounding the injection site was collected after 24. h. Analogous samples from untreated and PBS treated muscles served as controls. The analysis of genes expression using real-time PCR revealed significant up-regulation of proinflammatory cytokines: IL-1α, IL-1β, IL-6 in MSCs treated muscles in comparison to the PBS group. The evaluation of protein concentration (ELISA) in collected samples showed that injection of MSCs caused significant elevation of IL-1β. Immunofluorescent assessment of the tissue revealed infiltration of leukocytes and macrophages. Quantitative analysis of the samples immunostained for CD68 and CD43 antigens revealed that the number of phagocytes was significantly higher in MSC treated muscle when compared to the samples from PBS group. To conclude, the administration of mesenchymal stem cells into the muscle in syngeneic model induces the features of acute inflammation that affects cell engraftment.
机译：间充质干细胞由于具有高增殖潜力，多线粒分化能力成为再生医学的希望。然而，生物体对MSCs移植的反应没有完全阐明。本研究的目的是评估对Syngeneic MSCs给予肌肉的急性局部组织反应。将大鼠同胞MSC移植到骨骼肌中，24小时后收集注射部位周围的组织。来自未处理的类似样品和PBS处理的肌肉作为对照。使用实时PCR的基因表达的分析显示了促炎细胞因子的显着上调：IL-1α，IL-1β，IL-6与PBS组相比，MSC处理的肌肉。在收集的样品中对蛋白质浓度（ELISA）的评价显示，注射MSCs引起IL-1β的显着升高。组织的免疫荧光评估显示白细胞和巨噬细胞的浸润。与来自PBS组的样品相比，CD68和CD43抗原的样品的定量分析显示，MSC处理肌肉的吞噬细胞的数量显着高。为了得出结论，施用间充质干细胞进入时酯模型的肌肉诱导影响细胞植入的急性炎症的特征。

著录项

  • 来源
    《Cytokine》 |2013年第1期|共8页
  • 作者

    GalaK.; BurdzińskaA.; IdziakM.; WilczekE.; PaczekL.;

  • 作者单位

    Department of Immunology Transplantology and Internal Diseases Transplantation Institute Medical;

    Department of Immunology Transplantology and Internal Diseases Transplantation Institute Medical;

    Department of Immunology Transplantology and Internal Diseases Transplantation Institute Medical;

    Department of Pathology Medical University of Warsaw Poland;

    Department of Immunology Transplantology and Internal Diseases Transplantation Institute Medical;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Cytokines; Inflammation; Mesenchymal stem cells; Transplantation;

    机译：细胞因子;炎症;间充质干细胞;移植;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号