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首页> 外文期刊>Cytokine >Survival and immunomodulation of stem cells from human extracted deciduous teeth expanded in pooled human and foetal bovine sera
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Survival and immunomodulation of stem cells from human extracted deciduous teeth expanded in pooled human and foetal bovine sera

机译：来自人提取的落叶的干细胞的存活率和免疫调节在合并的人和胎牛血清中膨胀

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The immunomodulatory properties of mesenchymal stem cells (MSCs) from autologous and allogeneic sources are useful in stimulating tissue regeneration and repair. To obtain a high number of MSCs for transplantation requires extensive in vitro expansion with culture media supplements that can cause xeno-contamination of cells potentially compromising function and clinical outcomes. In this study stem cells from human extracted deciduous teeth (SHED) were cultured in Knockout (TM) DMEM supplemented with either pooled human serum (pHS) or foetal bovine serum (FBS) to compare their suitability in maintaining immunomodulatory properties of cells during in vitro expansion. No significant difference in cell survival of SHED grown in pHS (pHS-SHED) or FBS (FBS-SHED) was observed when co-cultured with complement, monocytes or lymphocytes. However, significant changes in the expression of sixteen paracrine factors involved in immunomodulation were observed in the supernatants of FBS-SHED co-cultures with monocytes or lymphocytes compared to that in pHS-SHEDs after both 24 and 120 h of incubation. Further analysis of changing protein levels of paracrine factors in co-cultures using biological pathway analysis software predicted upregulation of functions associated with immunogenicity in FBS-SHED and lymphocyte co-cultures compared to pHS-SHED co-cultures. Pathway analysis also predicted significant stimulation of HMGB1 and TREM1 signalling pathways in FBS-SHED co-cultures indicating activation of immune cells and inflammation. Though FBS supplementation does not impact survival of SHED, our combinatorial biological pathway analysis supports the idea that in vitro expansion of SHEDs in pHS provides optimal conditions to minimise xeno-contamination and inflammation and maintain their immunomodulatory properties.
机译：来自自体和同种异体来源的间充质干细胞（MSCs）的免疫调节性质可用于刺激组织再生和修复。为了获得大量的移植MSC，需要随着培养介质补充剂的广泛的体外膨胀，其可能导致卵泡污染的细胞可能损害功能和临床结果。在该研究中，来自人提取的落叶齿（SHED）的干细胞以淘汰的（TM）DMEM培养，所述淘汰的人血清（pHS）或胎牛血清（FBS）培养，以比较它们在体外期间保持细胞免疫调节性质的适用性扩张。当用补体，单核细胞或淋巴细胞共培养时，观察到在pHS（pHS-SHED）或FBS（FBS静脉）中生长的细胞存活的显着差异。然而，在用单核细胞或淋巴细胞中，在24和120小时的pHS-Sheds中，在FBS-Shed共培养物的上清液中观察到涉及免疫调节中涉及免疫调节的十六个旁曲静脉因子的表达的显着变化。进一步分析使用生物途径分析软件使用生物途径分析软件在共培养物中改变蛋白质水平的蛋白质水平预测与PHS-SHED和淋巴细胞共培养物中的免疫原性相关的功能的上调。途径分析还预测了在非本地血统共培养物中的HMGB1和Trem1信号通路对表明免疫细胞和炎症的激活的显着刺激。虽然FBS补充不影响SHED的存活，但我们的组合生物途径分析支持的想法，即PHS中棚的体外膨胀提供最佳条件，以最大限度地减少卵黄污染和炎症并保持其免疫调节性能。

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