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首页> 外文期刊>Cytokine >Genetic polymorphism rs8193036 of IL17A is associated with increased susceptibility to pulmonary tuberculosis in Chinese Han population
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Genetic polymorphism rs8193036 of IL17A is associated with increased susceptibility to pulmonary tuberculosis in Chinese Han population

机译：IL17A的遗传多态性RS8193036与中国汉族人群的肺结核易感性增加有关

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摘要

Th17 cells play a key role in immunity against Mycobacterium tuberculosis, our previous research showed that reduced Th17 responses were associated with the severe outcome of Mtb infection. The associations between IL17A polymorphisms and susceptibility of TB has been reported, but the results are inconsistent and the underlying mechanisms is unknown. In this study, we identified a genetic variation (rs8193036) in the promoter region of IL17A is associated with susceptibility to TB. The minor allele T frequency of rs8193036 was significantly different between patients with active TB (29.7%) and healthy controls (32.3%) (OR = 0.81; 95%CI, 0.71-0.93; P = 0.0026). Peripheral blood mononuclear cells from individuals carrying rs8193036CC genotypes produced significantly lower amount of IL17A upon CD3/28 stimulation compared to the individuals carrying rs8193036TT genotypes. Functional assay by reporter luciferase activity and EMSA demonstrated that rs8193036C exhibited significantly lower promotor transcription activities. In conclusion, our study confirmed that IL17A (rs8193036) is a functional SNP that could regulate gene expression though influencing transcription factor binding activity.
机译：Th17细胞在对结核分枝杆菌的免疫力发挥关键作用，我们以前的研究表明，减少的Th17反应与MTB感染的严重结果有关。报道了IL17A多态性与TB的易感性之间的关联，但结果不一致，潜在机制是未知的。在该研究中，我们鉴定了IL17A的启动子区域中的遗传变异（RS8193036）与TB的易感性相关。活性TB（29.7％）和健康对照（32.3％）（或= 0.81; 95％CI，0.71-0.93; P = 0.0026）之间的次要等位基因T频率在显着差异来自携带RS819303639CC基因型的个体的外周血单核细胞在CD3 / 28刺激上产生显着较低的IL17A，与携带RS8193036TT基因型的个体相比。记者荧光素酶活性和EMSA的功能测定证明RS8193036C表现出显着降低的促进转录活性。总之，我们的研究证实，IL17A（RS8193036）是一种功能性SNP，其可以调节基因表达，但不影响转录因子结合活性。

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  • 来源
    《Cytokine》 |2020年第2020期|共7页
  • 作者

    Wang Wenfei; Deng Guofang; Zhang Guoliang; Yu Ziqi; Yang Fan; Chen Jianyong; Cai Yi; Werz Oliver; Chen Xinchun;

  • 作者单位

    Shenzhen Univ Sch Med Dept Pathogen Biol Shenzhen Peoples R China;

    Shenzhen Third Peoples Hosp Shenzhen Key Lab Infect &

    Immun Guangdong Key Lab Emerging Infect Dis;

    Shenzhen Third Peoples Hosp Shenzhen Key Lab Infect &

    Immun Guangdong Key Lab Emerging Infect Dis;

    Nanchang Univ Jiangxi Med Coll Nanchang Jiangxi Peoples R China;

    Shenzhen Univ Sch Med Dept Pathogen Biol Shenzhen Peoples R China;

    Nanchang Univ Jiangxi Med Coll Nanchang Jiangxi Peoples R China;

    Shenzhen Univ Sch Med Dept Pathogen Biol Shenzhen Peoples R China;

    Friedrich Schiller Univ Inst Pharm Dept Pharmaceut Med Chem Jena Germany;

    Shenzhen Univ Sch Med Dept Pathogen Biol Shenzhen Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    IL17A; Polymorphism; Tuberculosis; Genotype; Therapy;

    机译：IL17A;多态性;结核病;基因型;治疗;

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