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首页> 外文期刊>Acta Neurochirurgica >Bacterial colonisation of suture material after routine neurosurgical procedures: relevance for wound infection
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Bacterial colonisation of suture material after routine neurosurgical procedures: relevance for wound infection

机译：常规神经外科手术后缝合材料的细菌定植：伤口感染的相关性

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Abstract Background Wound healing impairment is a serious problem in surgical disciplines which may be associated with chronic morbidity, increased cost and patient discomfort. Here we aimed to investigate the relevance of bacterial colonisation on suture material using polymerase chain reaction (PCR) to detect and taxonomically classify bacterial DNA in patients with and without wound healing problems after routine neurosurgical procedures. Methods Repeat surgery was performed in 25 patients with wound healing impairment and in 38 patients with well-healed wounds. To determine the presence of bacteria, a 16S rDNA-based PCR detection method was applied. Fragments of 500?bp were amplified using universal primers which target hypervariable regions within the bacterial 16S rRNA gene. Amplicons were separated from each other by single-strand conformation polymorphism (SSCP) analysis, and finally classified using Sanger sequencing. Results PCR/SSCP detected DNA of various bacteria species on suture material in 10/38 patients with well-healed wounds and in 12/25 patients with wound healing impairment including Staphylococcus aureus , Staphylococcus epidermidis , Propionibacterium acnes and Escherichia coli . Microbiological cultures showed bacterial growth in almost all patients with wound healing impairment and positive results in PCR/SSCP (10/12), while this was the case in only one patient with a well-healed wound (1/10). Conclusions Colonisation of suture material with bacteria occurs in a relevant portion of patients with and without wound healing impairment after routine neurosurgical procedures. Suture material may provide a nidus for bacteria and subsequent biofilm formation. Most likely, however, such colonisation of sutures is not a general primer for subsequent wound infection.
机译：摘要背景伤口愈合损伤是手术学科的严重问题，可能与慢性发病率相关，增加成本和患者的不适。在这里，我们旨在探讨使用聚合酶链反应（PCR）对细菌定植对缝合材料的相关性，以检测和分类在常规神经外科手术后患者患者患者中的细菌DNA。方法在25例伤口愈合障碍患者中进行重复手术，并在38例患有愈合良好的伤口患者中进行。为了确定细菌的存在，施加了一种16S的基于RDNA的PCR检测方法。使用靶向细菌16s rRNA基因内的高变区域的通用引物扩增500℃的片段。通过单链构象多态性（SSCP）分析彼此分离扩增子，并使用Sanger测序进行分类。结果PCR / SSCP在10/38患者中检测到各种细菌物种的DNA，在10/38患者中患有良好愈合的伤口，12/25伤口愈合损伤患者，包括金黄色葡萄球菌，葡萄球菌，葡萄球菌，丙酸杆菌和大肠杆菌。微生物培养物在PCR / SSCP（10/12）中几乎所有伤口愈合障碍患者的细菌生长和阳性结果，而这是只有一个患者患有愈合良好的伤口（1/10）的情况。结论在常规神经外科手术后患者的相关部分和没有伤口愈合损伤的相关部分中发生缝合材料的定植。缝合材料可为细菌和随后的生物膜形成提供滋养水。然而，最有可能的这种缝合线的定子不是用于随后伤口感染的一般底漆。

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