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首页> 外文期刊>Acta Poloniae Pharmaceutica: Durg Research >IN VITRO EVALUATION OF BIOLOGICAL ACTIVITY OF CINNAMIC, CAFFEIC, FERULIC AND CHLOROGENIC ACIDS WITH USE OF ESCHERICHIA COLI K-12 RECA::GFP BIOSENSOR STRAIN
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IN VITRO EVALUATION OF BIOLOGICAL ACTIVITY OF CINNAMIC, CAFFEIC, FERULIC AND CHLOROGENIC ACIDS WITH USE OF ESCHERICHIA COLI K-12 RECA::GFP BIOSENSOR STRAIN

机译:使用大肠杆菌K-12 Rega :: GFP生物传感器菌株的肉桂,咖啡酸,阿魏纤维和绿原酸生物活性的体外评价

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摘要

Cinnamic acid and its derivatives are important and promising compounds in cancer therapy, because of its broad spectrum of anicancer and antioxidative ability, and with high potential for development into new generation drugs. The aim of this study was to compare the cyto- and genotoxic effects of cinnamic acid and its derivatives with the use of Escherichia coli K-12 recA::gfp microbial biosensor strain with plasmid fusion of recA promoter and gfp gene as reporter. Obtained results indicate that recA::gfpmut2 genetic system was a sensitive biosensor to the most chemicals tested in our experiments. The cinnamic acid and its derivatives modulated the reactivity of recA promoter in relation to control sample and significantly inhibited bacteria cells growth. In the light of our results only chlorogenic and ferulic acids at higher concentrations demonstrated cyto-and genotoxic activity toward to E. coli K-12 recA::gfp cells.
机译:肉桂及其衍生物是癌症治疗中的重要且有希望的化合物,因为它具有广泛的基础探测剂和抗氧化能力,并且具有高潜力的发展进入新一代药物。 本研究的目的是将肉桂酸及其衍生物的使用与大肠杆菌K-12 RECA :: GFP微生物生物传感器菌株进行比较,并用RECA启动子和GFP基因作为记者的质粒融合。 获得的结果表明,RECA :: GFPMUT2遗传系统是我们实验中测试的最敏感的生物传感器。 肉桂酸及其衍生物调节RECA启动子与对照样品相关的反应性,并显着抑制细菌细胞生长。 鉴于我们的结果,较高浓度下的绿原和阿魏酸将在大肠杆菌K-12 Reca :: GFP细胞中表现出细胞和基因毒性活性。

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