...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta Veterinaria Hungarica >DISTINCT EXPRESSION PROFILE AND HISTOLOGICAL DISTRIBUTION OF NLRP3 INFLAMMASOME COMPONENTS IN THE TISSUES OF HAINAN BLACK GOAT SUGGEST A SITE-SPECIFIC ROLE IN THE INFLAMMATORY RESPONSE
【24h】

DISTINCT EXPRESSION PROFILE AND HISTOLOGICAL DISTRIBUTION OF NLRP3 INFLAMMASOME COMPONENTS IN THE TISSUES OF HAINAN BLACK GOAT SUGGEST A SITE-SPECIFIC ROLE IN THE INFLAMMATORY RESPONSE

机译：海南黑山羊组织中NLRP3炎症组分的明显表达谱和组织学分布表明炎症反应中的特异性作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The NOD-like receptor protein 3 (NLRP3) inflammasome comprised of NLRP3, ASC and caspase-1 plays an important role in the inflammatory and innate immune response. However, little is known about the expression pattern and histological distribution of these genes in goat. Here, we first cloned the full-length cDNAs of the NLRP3, ASC and caspase-1 genes of Hainan black goat and produced their polyclonal antibodies. Tissue-specific expression and histological distribution of these genes were analysed. Phylogenetic analysis revealed that these three goat genes had high homology with Bos taurus genes and low homology with avian or fish genes. After immunisations with these recombinant His-tagged proteins, the titres of antiserum were higher than 1: 1024 and purified IgG was obtained. These three genes were expressed in all examined tissues, the mRNA expression level of NLRP3 and caspase-1 was most abundant in the spleen and mesenteric lymph nodes (MLNs), while ASC was primary expressed in the liver, spleen and kidney. The histological distribution of NLRP3, ASC and caspase-1 was detected in myocardial cells, hepatocytes, focal lymphocytes, bron-chiolar epithelial cells, renal tubular epithelial cells, cortical neurons and endothelial cells of the germinal centres in the MLNs. These results will be helpful in further investigations into the function of the NLRP3 inflammasome and in elucidating its role in caprine inflammatory diseases.
机译：NOD样受体蛋白3（NLRP3）由NLRP3，ASC和Caspase-1组成的炎性组织在炎症和先天免疫应答中起重要作用。然而，关于这些基因在山羊中的表达模式和组织学分布很少。在这里，我们首先克隆了海南黑山羊的NLRP3，ASC和Caspase-1基因的全长CDNA，并产生了它们的多克隆抗体。分析了这些基因的组织特异性表达和组织学分布。系统发育分析表明，这三种山羊基因与博斯金牛群基因和禽类或鱼类基因的低同源性具有高同源性。在用这些重组的其标记蛋白免疫后，抗血清的滴度高于1：1024，得到纯化的IgG。在所有检查的组织中表达了这三个基因，NLRP3和Caspase-1的mRNA表达水平在脾脏和肠系膜淋巴结（MLNS）中最丰富，而ASC在肝脏，脾脏和肾脏中表达。在心肌细胞，肝细胞，局灶性淋巴细胞，支正上皮细胞，肾小管上皮细胞，皮质神经元和MLNS中生发中心的内皮细胞中检测到NLRP3，ASC和Caspase-1的组织学分布。这些结果将有助于进一步调查NLRP3炎症的功能，并阐明其在己碱炎性疾病中的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号