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首页> 外文期刊>Crystal growth & design >Polyhedral instability of glucose isomerase crystals as revealed by confocal scanning fluorescence microscopy
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Polyhedral instability of glucose isomerase crystals as revealed by confocal scanning fluorescence microscopy

机译：共焦扫描荧光显微镜显示的葡萄糖异构酶晶体的多面体不稳定性

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摘要

The polyhedral stability of a crystal during its growth plays a crucial role in determining its final quality. The polyhedral instability of glucose isomerase crystals in the presence of poly(ethylene glycol) (PEG) was observed using confocal scanning fluorescence microscopy, which enabled in situ visualization and real-time determination of the variation of protein concentration around the growing crystal. The presence of a zone depleted in protein around the crystal shows that the observed polyhedral instability occurred under diffusion-limited conditions and so was governed mainly by the supersaturation, the crystal size, and the solution viscosity. The polyhedral instability of protein crystals therefore shares some common features with the instability of crystals of inorganic or small organic molecules.
机译：晶体在生长过程中的多面体稳定性在决定其最终质量方面起着至关重要的作用。使用共聚焦扫描荧光显微镜观察了在存在聚乙二醇（PEG）的情况下葡萄糖异构酶晶体的多面体不稳定性，该方法能够在原位观察并实时确定生长晶体周围蛋白质浓度的变化。晶体周围蛋白质耗尽的区域的存在表明，观察到的多面体不稳定性是在扩散受限的条件下发生的，因此主要由过饱和度，晶体尺寸和溶液粘度决定。因此，蛋白质晶体的多面体不稳定性与无机或有机小分子晶体的不稳定性具有一些共同的特征。

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