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首页> 外文期刊>ACS Sustainable Chemistry & Engineering >Field-Grown Transgenic Hybrid Poplar with Modified Lignin Biosynthesis to Improve Enzymatic Saccharification Efficiency
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Field-Grown Transgenic Hybrid Poplar with Modified Lignin Biosynthesis to Improve Enzymatic Saccharification Efficiency

机译：具有改性木质素生物合成的现场生长的转基因杂交杨杨，提高酶糖化效率

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摘要

Hybrid poplars (Populus nigra L. X Populus maximowiczii A.) were genetically modified through antisense insertion of the 4-coumarate:coenzyme A ligase (4CL) gene. Compositional changes in response to this genetic change were measured in the field after 2 and 3 years of growth. The stem samples were treated with either green liquor or dilute acid pretreatments, representing alkaline and acid pretreatments. The enzymatic saccharification of the untreated and pretreated transgenic poplars were evaluated. After transgenic species were transplanted into the environment, they showed reduced recalcitrance to chemicals (i.e., pretreatments) and enzymes despite their lignin content and S/V ratio being comparable to those of the wild types. Compared to the field-grown poplars, the sugar yield increased up to 103% for untreated transgenic samples and increased 22% for acid- and green liquor-pretreated transgenic samples. This shows that field-grown transgenic hybrid poplars with modified lignin biosynthesis have improved enzymatic saccharification efficiency (sugar recovery and yield).
机译：杂交杨树（杨树NIGRA L. x Populus MaximoWiCzii A.）通过反义插入4-作用的插入遗传修饰：辅酶A连接酶（4CL）基因。在2至3年的生长后，在田间测量响应于该遗传变化的组成变化。用绿液或稀酸预处理处理茎样品，代表碱性和酸预处理。评价未处理和预处理的转基因杨树的酶糖化。在转基因物种移植到环境中后，它们表明，尽管它们的木质素含量和S / V比与野生类型的比例相当，但它们表明对化学物质（即预处理）和酶的批量分析。与现场生长的杨树相比，未处理的转基因样品的糖产率增加了103％，酸和绿液预处理的转基因样品增加了22％。这表明，具有改性木质素生物合成的现场生长的转基因杂交杨蛋白具有改善的酶糖化效率（糖恢复和产量）。

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