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首页> 外文期刊>ACS Sustainable Chemistry & Engineering >Pillar[6]arene@AuNPs Functionalized N-CQDs@Co3O4 Hybrid Composite for Ultrasensitive Electrochemical Detection of Human Epididymis Protein 4
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Pillar[6]arene@AuNPs Functionalized N-CQDs@Co3O4 Hybrid Composite for Ultrasensitive Electrochemical Detection of Human Epididymis Protein 4

机译：柱[6]芳烃@ AUNPS功能化N-CQDS @ CO3O4杂交复合材料，用于超敏感的电化学检测人体附睾蛋白4

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摘要

Hydroxylatopillar[6]arene (HP6) has been used as a stable ligand for the synthesis of gold nanoparticles (AuNPs). HP6 with excellent molecular recognition capability will securely capture the electron transfer medium thionin (Thi). Compared with other electroactive molecular capture methods (physical adsorption, etc.), the formation of an HP6 superset of Thi complex can effectively inhibit the leakage of Thi and significantly increase the loading of Thi. HP6@Au was prepared in an aqueous solution with OH- at room temperature through an oxidation-reduction reaction between HP6 and AuCl4-, without the need for conventional harsh reducing agents. X-ray photoelectron spectroscopy and Fourier transform infrared studies have shown that the -OH groups in HP6 are oxidized to -COOH groups, and Au3+ in AuCl4- is reduced to Au-0, leading to nucleation and production of AuNPs. The formation of HP6@Au strengthens the electron transfer rate, increases surface area, and captures a large number of primary antibodies (Ab1). Besides, the surface of N-doped carbon quantum dots and cobalt oxide (N-CQDs@Co3O4) can be used for the deposition of HP6@Au by pi-pi interaction to form a green highly conductive nanocomposite. An electrochemical immunosensor with significant stability was constructed based on HP6@Au/N-CQDs@Co3O4, and it was used for the effective detection of human epididymis protein 4 (HE4). The detection limit of the proposed immunosensor platform is 1.5 pg mL(-1), and it provides a wide linear range from 0.002 to 20 ng mL(-1) under optimal conditions. The analysis results of HE4 in human serum are satisfactory and have broad application prospects in clinical monitoring.
机译：羟基吡咯[6]芳烃（HP6）被用作合成金纳米颗粒（AUNP）的稳定配体。具有优异的分子识别能力的HP6将牢固地捕获电子转移介质Thionin（THI）。与其他电活性分子捕获方法（物理吸附等）相比，Thi复合物的HP6超集的形成可以有效地抑制THI的泄漏并显着增加THI的负荷。通过HP6和AuCl4-的氧化还原反应，在室温下用OH-在室温下制备HP6 @ Au，而无需常规的苛刻还原剂。 X射线光电子能谱和傅里叶变换红外研究表明，HP6中的-OH基团氧化成-OOH基团，AuCl4中的Au3 +降至Au-0，导致患者的成核和产生。 HP6 @ Au的形成增强了电子传递速率，增加了表面积，并捕获了大量的一抗（AB1）。此外，N掺杂的碳量子点和氧化钴（N-CQDS @ CO3O4）的表面可用于通过PI-PI相互作用沉积HP6 @ Au，形成绿色高导电纳米复合材料。基于HP6 / N-CQDS @ CO3O4构建具有显着稳定性的电化学免疫传感器，用于有效检测人对抗蛋白4（HE4）。所提出的免疫传感器平台的检测极限为1.5pg ml（-1），在最佳条件下提供0.002至20ng ml（-1）的宽线性范围。人血清中HE4的分析结果令人满意，在临床监测中具有广泛的应用前景。

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