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首页> 外文期刊>ACS Sustainable Chemistry & Engineering >A Vault-Encapsulated Enzyme Approach for Efficient Degradation and Detoxification of Bisphenol A and Its Analogues
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A Vault-Encapsulated Enzyme Approach for Efficient Degradation and Detoxification of Bisphenol A and Its Analogues

机译：一种封装的酶方法，用于高效降解和双酚A及其类似物的解毒

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摘要

We report an effective and environmentally sustainable water treatment approach using enzymes encapsulated in biogenic vault nanoparticles. Manganese peroxidase (MnP), whose stability was remarkably extended by encapsulating into vaults, rapidly catalyzed the biotransformation of endocrine-disrupting compounds, including bisphenol A (BPA), bisphenol F (BPF), and bisphenol AP (BPAP). The vault-encapsulated MnP (vMnP) treatment removed 80-95% of each of the tested bisphenols (BPs) at lower enzyme dosage, while free native MnP (nMnP) only resulted in a 19-36% removal, over a 24-h period. Treatment by vMnP and nMnP resulted in considerable disparities in product species and abundance, which were consistent with the observed changes in the estrogenic activities of BPs. To test if vMnP-catalyzed transformations generated toxic intermediates, we assessed biological hallmarks of BP toxicity, namely, the ability to disrupt reproductive processes. The toxicity of vMnPtreated samples, as measured in the model organism, Caenorhabditis elegans, was dramatically reduced for all three BPs, including the reproductive indicators of BPA exposure such as reduced fertility and increased germ cell death. Collectively, our results indicate that the vMnP system represents an efficient and safe approach for the removal of BPs and promise the development of vault-encapsulated customized enzymes for treating other targeted organic compounds in contaminated waters.
机译：我们通过在生物拱柱纳米颗粒中包封的酶报告了一种有效和环境可持续的水处理方法。锰过氧化物酶（MNP），其稳定性通过包封在拱顶中显着延伸，迅速催化了内分泌破坏化合物的生物转化，包括双酚A（BPA），双酚F（BPF）和双酚AP（BPAP）。覆盖的MNP（VMNP）处理除以较低酶剂量的80-95％的每种测试的双酚（BPS），而自由天然的MNP（NMNP）仅导致19-36％的去除，超过24小时时期。 VMNP和NMNP的处理导致产品物种和丰度具有相当大的差异，这与观察到的BPS雌激素活性的变化一致。为了测试VMNP催化的转化产生有毒中间体，我们评估了BP毒性的生物标志，即扰乱生殖过程的能力。在模型生物中，在模型生物中测量的所有三个BPS测量的Vmnptreated样品的毒性大大降低了BPA暴露的生殖指标，如降低生育率和胚芽细胞死亡。统称，我们的结果表明，VMNP系统代表了去除BPS的有效和安全的方法，并承诺将保险库包封的定制酶的发展用于治疗污染水中的其他靶向有机化合物。

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